ArticleMethods in molecular biology (Clifton, N.J.)2015
Expressing and characterizing mechanosensitive channels in Xenopus oocytes.
Article in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Biological fundamental functional units of meridians: mechanosensitive ion channels.Frontiers in medicine · 2026Review
- ALMT12 interacts with and inhibits SLAC1 to modulate stomatal movements and enhance plant biomass.Plant physiology · 2025Article
- Picrotoxin Delineates Different Transport Configurations for Malate and γ Aminobutyric Acid through TaALMT1.Biology · 2022Article
- OzTracs: Optical Osmolality Reporters Engineered from Mechanosensitive Ion Channels.Biomolecules · 2022Article
- Fluorescence lifetime imaging reveals heterogeneous functional distribution of eGFP expressed in Xenopus oocytes.Methods and applications in fluorescence · 2019Article
- Nonpolar residues in the presumptive pore-lining helix of mechanosensitive channel MSL10 influence channel behavior and establish a nonconducting function.Plant direct · 2018Article
- OsALMT7 Maintains Panicle Size and Grain Yield in Rice by Mediating Malate Transport.The Plant cell · 2018Article
- Expanding the genetic toolkit in Xenopus: Approaches and opportunities for human disease modeling.Developmental biology · 2017Review
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2 authors.
Funding
Abstract
The oocytes of the African clawed frog (Xenopus laevis) comprise one of the most widely used membrane protein expression systems. While frequently used for studies of transporters and ion channels, the application of this system to the study of mechanosensitive ion channels has been overlooked, perhaps due to a relative abundance of native expression systems. Recent advances, however, have illustrated the advantages of the oocyte system for studying plant and bacterial mechanosensitive channels. Here we describe in detail the methods used for heterologous expression and characterization of bacterial and plant mechanosensitive channels in Xenopus oocytes.
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