ArticleMagnetic resonance in medicine2016
Development of Timd2 as a reporter gene for MRI.
Article in Magnetic resonance in medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
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- CT-Visible Microspheres Enable Whole-Body In Vivo Tracking of Injectable Tissue Engineering Scaffolds.Advanced healthcare materials · 2024Article
- A Dual-Gene Reporter-Amplifier Architecture for Enhancing the Sensitivity of Molecular MRI by Water Exchange.Chembiochem : a European journal of chemical biology · 2024Article
- Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types.Journal of biological engineering · 2024Article
- A dual-gene reporter-amplifier architecture for enhancing the sensitivity of molecular MRI by water exchange.bioRxiv : the preprint server for biology · 2024Article
- Exploring the potential of water channels for developing MRI reporters and sensors without the need for exogenous contrast agents.bioRxiv : the preprint server for biology · 2024Article
- Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types.bioRxiv : the preprint server for biology · 2023Article
- Biogenic Imaging Contrast Agents.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Optical and MRI Multimodal Tracing of Stem Cells In Vivo.Molecular imaging · 2023Review
- Designing Protein-Based Probes for Sensing Biological Analytes with Magnetic Resonance Imaging.Analysis & sensing · 2022Article
- Application of Genetically Encoded Molecular Imaging Probes in Tumor Imaging.Contrast media & molecular imaging · 2022Review
- A brief review of reporter gene imaging in oncolytic virotherapy and gene therapy.Molecular therapy oncolytics · 2021Review
- Beyond the Green Fluorescent Protein: Biomolecular Reporters for Anaerobic and Deep-Tissue Imaging.Bioconjugate chemistry · 2020Review
- Stem Cell Tracing Through MR Molecular Imaging.Tissue engineering and regenerative medicine · 2018Review
- Characterization of Magneto-Endosymbionts as MRI Cell Labeling and Tracking Agents.Molecular imaging and biology · 2018Article
- Biomolecular MRI reporters: Evolution of new mechanisms.Progress in nuclear magnetic resonance spectroscopy · 2017Review
- Molecular Imaging in Synthetic Biology, and Synthetic Biology in Molecular Imaging.Molecular imaging and biology · 2017Review
- Non-invasive imaging using reporter genes altering cellular water permeability.Nature communications · 2016Article
- Magnetic hyperthermia controlled drug release in the GI tract: solving the problem of detection.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo assess the potential of an MRI gene reporter based on the ferritin receptor Timd2 (T-cell immunoglobulin and mucin domain containing protein 2), using T1- and T2-weighted imaging.
methodsPellets of cells that had been modified to express the Timd2 transgene, and incubated with either iron-loaded or manganese-loaded ferritin, were imaged using T1- and T2-weighted MRI. Mice were also implanted subcutaneously with Timd2-expressing cells and the resulting xenograft tissue imaged following intravenous injection of ferritin using T2-weighted imaging.
resultsTimd2-expressing cells, but not control cells, showed a large increase in both R2 and R1 in vitro following incubation with iron-loaded and manganese-loaded ferritin, respectively. Expression of Timd2 had no effect on cell viability or proliferation; however, manganese-loaded ferritin, but not iron-loaded ferritin, was toxic to Timd2-expressing cells. Timd2-expressing xenografts in vivo showed much smaller changes in R2 following injection of iron-loaded ferritin than the same cells incubated in vitro with iron-loaded ferritin.
conclusionTimd2 has demonstrated potential as an MRI reporter gene, producing large increases in R2 and R1 with ferritin and manganese-loaded ferritin respectively in vitro, although more modest changes in R2 in vivo. Manganese-loaded apoferritin was not used in vivo due to the toxicity observed in vitro. Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance.
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