ReviewCurrent genetics2015
High-throughput cell-cycle imaging opens new doors for discovery.
Review in Current genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed.
- OmniSegger: A time-lapse image analysis pipeline for bacterial cells.PLoS computational biology · 2025Article
- The Quantification of Bacterial Cell Size: Discrepancies Arise from Varied Quantification Methods.Life (Basel, Switzerland) · 2023Article
- Fundamental principles in bacterial physiology-history, recent progress, and the future with focus on cell size control: a review.Reports on progress in physics. Physical Society (Great Britain) · 2018Review
- Ribosome profiling the cell cycle: lessons and challenges.Current genetics · 2017Review
- Connecting virulence pathways to cell-cycle progression in the fungal pathogen Cryptococcus neoformans.Current genetics · 2017Review
- Transcriptomic analysis displays the effect of (-)-roemerine on the motility and nutrient uptake in Escherichia coli.Current genetics · 2017Article
- Probing bacterial cell biology using image cytometry.Molecular microbiology · 2017Article
- Cytoplasmic RNA-Protein Particles Exhibit Non-Gaussian Subdiffusive Behavior.Biophysical journal · 2017Article
- Using the power of genetic suppressors to probe the essential functions of RNase E.Current genetics · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the life of a cell, numerous essential cellular processes must be coordinated both spatially and temporally, from DNA replication and chromosome segregation to gene expression and cytokinesis. In order to analyze these inherently dynamic and cell-cycle-dependent processes, it is essential to observe the dynamic localization of the cellular machinery throughout the entire cell cycle. Although some coarse features of cell-cycle dynamics can be captured in snapshot imaging, where cellular size or morphology can be used as a proxy for cell-cycle phase, the inherently stochastic nature of ultrastructures in the cell makes the direct visualization of subcellular dynamics an essential tool to differentiate between structural differences that are the result of biologically relevant dynamics versus cell-to-cell variation. With these goals in mind, we have developed a unique high-throughput imaging approach, and have recently applied this to characterize the cell-cycle localization of nearly every protein in the bacterial cell (Kuwada in Mol Microbiol, 95(1), 64-79, 2015). This approach combines large-format sample preparation with automated image capture, processing, and analysis to quantitatively characterize proteome localization of tens of thousands of complete cell cycles.
Indexed as
Identifiers
25980547What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.