ArticleNucleic acids research2015
De novo design of heat-repressible RNA thermosensors in E. coli.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- RNA thermometers as temperature-dependent tools for enhanced recombinant protein production in precision fermentation.Microbial cell factories · 2026Article
- Temperature-responsive regulation of the polycyclic aromatic hydrocarbon-degrading mesophilic bacteriumApplied and environmental microbiology · 2025Article
- Customizing cellular signal processing by synthetic multi-level regulatory circuits.Nature communications · 2023Review
- Developing New Tools to Fight Human Pathogens: A Journey through the Advances in RNA Technologies.Microorganisms · 2022Review
- Recent Synthetic Biology Approaches for Temperature- and Light-Controlled Gene Expression in Bacterial Hosts.Molecules (Basel, Switzerland) · 2022Review
- Tunable Temperature-Sensitive Transcriptional Activation Based on Lambda Repressor.ACS synthetic biology · 2022Article
- Genetically stable CRISPR-based kill switches for engineered microbes.Nature communications · 2022Article
- Cybergenetic control of microbial community composition.Frontiers in bioengineering and biotechnology · 2022Review
- Predictable control of RNA lifetime using engineered degradation-tuning RNAs.Nature chemical biology · 2021Article
- Biosensing in Smart Engineered Probiotics.Biotechnology journal · 2020Review
- A tight cold-inducible switch built by coupling thermosensitive transcriptional and proteolytic regulatory parts.Nucleic acids research · 2019Article
- Cell-Free Synthetic Biology Platform for Engineering Synthetic Biological Circuits and Systems.Methods and protocols · 2019Review
- Biomolecular Ultrasound and Sonogenetics.Annual review of chemical and biomolecular engineering · 2018Review
- RNA Thermometers for the PURExpress System.ACS synthetic biology · 2018Article
- Characterizing the Structure-Function Relationship of a Naturally Occurring RNA Thermometer.Biochemistry · 2017Article
- Engineering a riboswitch-based genetic platform for the self-directed evolution of acid-tolerant phenotypes.Nature communications · 2017Article
- Dynamics of sequestration-based gene regulatory cascades.Nucleic acids research · 2017Article
- Exploring the modular nature of riboswitches and RNA thermometers.Nucleic acids research · 2016Article
- RNAiFold2T: Constraint Programming design of thermo-IRES switches.Bioinformatics (Oxford, England) · 2016Article
- Designing synthetic RNAs to determine the relevance of structural motifs in picornavirus IRES elements.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA-based temperature sensing is common in bacteria that live in fluctuating environments. Most naturally-occurring RNA thermosensors are heat-inducible, have long sequences, and function by sequestering the ribosome binding site in a hairpin structure at lower temperatures. Here, we demonstrate the de novo design of short, heat-repressible RNA thermosensors. These thermosensors contain a cleavage site for RNase E, an enzyme native to Escherichia coli and many other organisms, in the 5' untranslated region of the target gene. At low temperatures, the cleavage site is sequestered in a stem-loop, and gene expression is unobstructed. At high temperatures, the stem-loop unfolds, allowing for mRNA degradation and turning off expression. We demonstrated that these thermosensors respond specifically to temperature and provided experimental support for the central role of RNase E in the mechanism. We also demonstrated the modularity of these RNA thermosensors by constructing a three-input composite circuit that utilizes transcriptional, post-transcriptional, and post-translational regulation. A thorough analysis of the 24 thermosensors allowed for the development of design guidelines for systematic construction of similar thermosensors in future applications. These short, modular RNA thermosensors can be applied to the construction of complex genetic circuits, facilitating rational reprogramming of cellular processes for synthetic biology applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.