ArticleAmerican journal of physiology. Lung cellular and molecular physiology2015
Endothelial disruptive proinflammatory effects of nicotine and e-cigarette vapor exposures.
Article in American journal of physiology. Lung cellular and molecular physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 147 papers, 4 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
147 citing papers in PubMed, 4 syntheses or guidelines pooled it, 259 citations in OpenAlex.
- Comparison of Acute Effects of E-cigarettes With and Without Nicotine and Tobacco Cigarettes on Hemodynamic and Endothelial Parameters: A Systematic Review and Meta-analysis.High blood pressure & cardiovascular prevention : the official journal of the Italian Society of Hypertension · 2024Pooled it
- E-cigarette use and respiratory disorders: an integrative review of converging evidence from epidemiological and laboratory studies.The European respiratory journal · 2021Pooled it
- Electronic nicotine delivery system use is related to higher odds of alcohol and marijuana use in adolescents: Meta-analytic evidence.Addictive behaviors · 2020Pooled it
- Overview of Electronic Nicotine Delivery Systems: A Systematic Review.American journal of preventive medicine · 2017Pooled it
- Altered lung biology of healthy never smokers following acute inhalation of E-cigarettes.Respiratory research · 2018Trial
- E-cigarette exposure triggers distal lung cell injury, persistent lung stress response, and antiviral immune suppression.JCI insight · 2026Article
- Reduced vascular function, ventilatory efficiency and exercise capacity in young adult electronic cigarette users.ERJ open research · 2026Article
- E-cigarette Aerosol Containing Nicotine Increases Aortic Stiffness in Young Mice.Cardiovascular toxicology · 2026Article
- To Vape or Not to Vape: Oral Health in the Age of Electronic Nicotine Delivery Systems and Electronic Non-Nicotine Delivery Systems.International dental journal · 2026Review
- Molecular Health Effects of Electronic Cigarettes.Biomolecules · 2026Review
- New-onset atrial fibrillation in a healthy young adult following electronic cigarette use.Journal of Taibah University Medical Sciences · 2026Article
- Cannabis and nicotine use are independently associated with adverse surgical, medical, and psychosocial outcomes following upper extremity fracture fixation.Journal of orthopaedic surgery and research · 2026Article
- Multisystem Toxicity of E-Cigarettes in Preclinical and Clinical Studies: Pathophysiologic Effects of E-Cigarette Aerosol Exposures from Head to Toe.Annual review of pharmacology and toxicology · 2026Review
- Nanomechanical Characterization of E-Cigarette-Induced Lung Endothelial Dysfunction: Roles of Cortactin and Mitochondrial Reactive Oxygen Species.International journal of molecular sciences · 2025Article
- Nicotinism vs. Glomerulopathies-Smoking as a Risk Factor for Primary Glomerulopathies.Antioxidants (Basel, Switzerland) · 2025Review
- Maternal Electronic Cigarette Exposure Induces Dysregulation of Autophagy via Oxidative Stress/DNA Methylation in Pulmonary Hypertension Offspring.Current medical science · 2025Article
- Global disease burden analysis of Cardiometabolic disease attributable to second-hand smoke exposure from 1990 to 2040.American journal of preventive cardiology · 2025Article
- Nicotine-free electronic vape fluid stimulates angiogenic processesFrontiers in toxicology · 2025Article
- Inflammatory response to chronic nicotine-containing electronic cigarette exposure in a rat model of myocardial infarction.Tobacco induced diseases · 2025Article
- E-Cigarettes induce expression of procoagulant tissue factor in cultivated human endothelial cells.Journal of thrombosis and thrombolysis · 2025Article
87 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors at 5 institutions in 1 country.
Funding
Abstract
The increased use of inhaled nicotine via e-cigarettes has unknown risks to lung health. Having previously shown that cigarette smoke (CS) extract disrupts the lung microvasculature barrier function by endothelial cell activation and cytoskeletal rearrangement, we investigated the contribution of nicotine in CS or e-cigarettes (e-Cig) to lung endothelial injury. Primary lung microvascular endothelial cells were exposed to nicotine, e-Cig solution, or condensed e-Cig vapor (1-20 mM nicotine) or to nicotine-free CS extract or e-Cig solutions. Compared with nicotine-containing extract, nicotine free-CS extract (10-20%) caused significantly less endothelial permeability as measured with electric cell-substrate impedance sensing. Nicotine exposures triggered dose-dependent loss of endothelial barrier in cultured cell monolayers and rapidly increased lung inflammation and oxidative stress in mice. The endothelial barrier disruptive effects were associated with increased intracellular ceramides, p38 MAPK activation, and myosin light chain (MLC) phosphorylation, and was critically mediated by Rho-activated kinase via inhibition of MLC-phosphatase unit MYPT1. Although nicotine at sufficient concentrations to cause endothelial barrier loss did not trigger cell necrosis, it markedly inhibited cell proliferation. Augmentation of sphingosine-1-phosphate (S1P) signaling via S1P1 improved both endothelial cell proliferation and barrier function during nicotine exposures. Nicotine-independent effects of e-Cig solutions were noted, which may be attributable to acrolein, detected along with propylene glycol, glycerol, and nicotine by NMR, mass spectrometry, and gas chromatography, in both e-Cig solutions and vapor. These results suggest that soluble components of e-Cig, including nicotine, cause dose-dependent loss of lung endothelial barrier function, which is associated with oxidative stress and brisk inflammation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.