Evidence map›Paper›PMID 25978622›Full record

ArticlePloS one2015

Sensitive detection of norovirus using phage nanoparticle reporters in lateral-flow assay.

Anna E V Hagström, Gavin Garvey, Andrew S Paterson, Sagar Dhamane, Meena Adhikari, Mary K Estes, Ulrich Strych, Katerina Kourentzi, Robert L Atmar, Richard C Willson

Abstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. Review
  7. Article
  8. A Survey of Analytical Techniques for Noroviruses.Foods (Basel, Switzerland) · 2020
    Review
  9. Article
  10. Article
  11. Review
  12. [Research advance of lateral flow assay labels].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2018
    Review
  13. Article
  14. Review
  15. Review
  16. Article
  17. Review
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Anna E V HagströmDepartment of Chemical and Biomolecular Engineering, University of Houston, Houston, Texas, United States of America.
Gavin GarveyDepartment of Chemical and Biomolecular Engineering, University of Houston, Houston, Texas, United States of America.
Andrew S PatersonDepartment of Chemical and Biomolecular Engineering, University of Houston, Houston, Texas, United States of America.
Sagar DhamaneDepartment of Biology and Biochemistry, University of Houston, Houston, Texas, United States of America.
Meena AdhikariDepartment of Biology and Biochemistry, University of Houston, Houston, Texas, United States of America.
Mary K EstesDepartment of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States of America; Department of Medicine, Baylor College of Medicine, Houston, Texas, United States of America.
Ulrich StrychDepartment of Biology and Biochemistry, University of Houston, Houston, Texas, United States of America.
Katerina KourentziDepartment of Chemical and Biomolecular Engineering, University of Houston, Houston, Texas, United States of America.
Robert L AtmarDepartment of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States of America; Department of Medicine, Baylor College of Medicine, Houston, Texas, United States of America.
Richard C WillsonDepartment of Chemical and Biomolecular Engineering, University of Houston, Houston, Texas, United States of America; Department of Biology and Biochemistry, University of Houston, Houston, Texas, United States of America; Houston Methodist Research Institute, Houston, Texas, United States of America; Tecnológico de Monterrey, Departamento de Biotecnología e Ingeniería de Alimentos, Centro de Biotecnología FEMSA, Monterrey, Nuevo León, Mexico.

Funding

WRCE Nonhuman Primate CoreU54AI057156 · NIAID · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI WALKER, DAVID H · 2003 to 2013
$102.6M
X-ray Crystallographic Studies on CalicivirusesP01AI057788 · NIAID · BAYLOR COLLEGE OF MEDICINE · PI ATMAR, ROBERT L., ESTES, MARY KOLB · 2004 to 2024
$34.4M
Tissue Analysis & Molecular Imaging CoreP30DK056338 · NIDDK · BAYLOR COLLEGE OF MEDICINE · PI Geoffrey A Preidis · 2001 to 2026
$28.3M
NIAID NIH HHS P01 AI057788NIAID NIH HHS U54 AI057156NIDDK NIH HHS P30 DK056338
6 · The paper itself

Abstract

Noroviruses are recognized worldwide as the principal cause of acute, non-bacterial gastroenteritis, resulting in 19-21 million cases of disease every year in the United States. Noroviruses have a very low infectious dose, a short incubation period, high resistance to traditional disinfection techniques and multiple modes of transmission, making early, point-of-care detection essential for controlling the spread of the disease. The traditional diagnostic tools, electron microscopy, RT-PCR and ELISA require sophisticated and expensive instrumentation, and are considered too laborious and slow to be useful during severe outbreaks. In this paper we describe the development of a new, rapid and sensitive lateral-flow assay using labeled phage particles for the detection of the prototypical norovirus GI.1 (Norwalk), with a limit of detection of 107 virus-like particles per mL, one hundred-fold lower than a conventional gold nanoparticle lateral-flow assay using the same antibody pair.

Indexed as

BacteriophagesBiological AssayCaliciviridae InfectionsEscherichia coliGastroenteritisHumansNanoparticlesNorovirusSensitivity and SpecificityUnited States

Identifiers

PMID25978622
PMCPMC4433186

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.