ArticleThe ISME journal2015
High molecular weight dissolved organic matter enrichment selects for methylotrophs in dilution to extinction cultures.
Article in The ISME journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 54 citations in OpenAlex.
- Oxidation state of bioavailable dissolved organic matter influences bacterioplankton respiration and growth efficiency.Communications biology · 2025Article
- Diversity in the utilization of different molecular classes of dissolved organic matter by heterotrophic marine bacteria.Applied and environmental microbiology · 2024Article
- Effect of ocean acidification on the growth, response and hydrocarbon degradation of coccolithophore-bacterial communities exposed to crude oil.Scientific reports · 2023Article
- Rapid bacterioplankton transcription cascades regulate organic matter utilization during phytoplankton bloom progression in a coastal upwelling system.The ISME journal · 2022Article
- Article
- Organic Matter Composition at Ocean Station Papa Affects Its Bioavailability, Bacterioplankton Growth Efficiency and the Responding Taxa.Frontiers in Marine Science · 2021Article
- Size-Fractionated Microbiome Structure in Subarctic Rivers and a Coastal Plume Across DOC and Salinity Gradients.Frontiers in microbiology · 2021Article
- Mle046 Is a Marine Mesophilic MHETase-Like Enzyme.Frontiers in microbiology · 2021Article
- Molecular Basis for Substrate Recognition and Catalysis by a Marine Bacterial Laminarinase.Applied and environmental microbiology · 2020Article
- Expanding the Diversity of Bacterioplankton Isolates and Modeling Isolation Efficacy with Large-Scale Dilution-to-Extinction Cultivation.Applied and environmental microbiology · 2020Article
- Construction of Simplified Microbial Consortia to Degrade Recalcitrant Materials Based on Enrichment and Dilution-to-Extinction Cultures.Frontiers in microbiology · 2019Article
- Distinct Coastal Microbiome Populations Associated With Autochthonous- and Allochthonous-Like Dissolved Organic Matter.Frontiers in microbiology · 2019Article
- Microbial communities of aquatic environments on Heard Island characterized by pyrotag sequencing and environmental data.Scientific reports · 2017Article
- Molecular Fractionation of Dissolved Organic Matter in a Shallow Subterranean Estuary: The Role of the Iron Curtain.Environmental science & technology · 2017Article
- Isolation and Characterization of Bacteria That Degrade Phosphonates in Marine Dissolved Organic Matter.Frontiers in microbiology · 2017Article
- Functional Gene Diversity and Metabolic Potential of the Microbial Community in an Estuary-Shelf Environment.Frontiers in microbiology · 2017Article
- Interkingdom Cross-Feeding of Ammonium from Marine Methylamine-Degrading Bacteria to the Diatom Phaeodactylum tricornutum.Applied and environmental microbiology · 2016Article
- Article
- Deciphering ocean carbon in a changing world.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Expansion of Cultured Bacterial Diversity by Large-Scale Dilution-to-Extinction Culturing from a Single Seawater Sample.Microbial ecology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of bacterioplankton in the cycling of marine dissolved organic matter (DOM) is central to the carbon and energy balance in the ocean, yet there are few model organisms available to investigate the genes, metabolic pathways, and biochemical mechanisms involved in the degradation of this globally important carbon pool. To obtain microbial isolates capable of degrading semi-labile DOM for growth, we conducted dilution to extinction cultivation experiments using seawater enriched with high molecular weight (HMW) DOM. In total, 93 isolates were obtained. Amendments using HMW DOM to increase the dissolved organic carbon concentration 4x (280 μM) or 10x (700 μM) the ocean surface water concentrations yielded positive growth in 4-6% of replicate dilutions, whereas <1% scored positive for growth in non-DOM-amended controls. The majority (71%) of isolates displayed a distinct increase in cell yields when grown in increasing concentrations of HMW DOM. Whole-genome sequencing was used to screen the culture collection for purity and to determine the phylogenetic identity of the isolates. Eleven percent of the isolates belonged to the gammaproteobacteria including Alteromonadales (the SAR92 clade) and Vibrio. Surprisingly, 85% of isolates belonged to the methylotrophic OM43 clade of betaproteobacteria, bacteria thought to metabolically specialize in degrading C1 compounds. Growth of these isolates on methanol confirmed their methylotrophic phenotype. Our results indicate that dilution to extinction cultivation enriched with natural sources of organic substrates has a potential to reveal the previously unsuspected relationships between naturally occurring organic nutrients and the microorganisms that consume them.
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