ArticlePloS one2015
The Intraperitoneal Transcriptome of the Opportunistic Pathogen Enterococcus faecalis in Mice.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 44 citations in OpenAlex.
- Functional redundancy of translation-associated factors masks the role of YebC2 in Enterococcus faecalis.Folia microbiologica · 2026Article
- Metal uptake systems underpinInfection and immunity · 2026Article
- Wound-Healing Potential of Engineered Lysin GRC-ML07 inAntibiotics (Basel, Switzerland) · 2025Article
- The NagY antiterminator inMicrobiology spectrum · 2025Article
- Article
- Cellodextrin Metabolism and Phosphotransferase System-Catalyzed Uptake in Enterococcus faecalis.Molecular microbiology · 2025Article
- Insights into ecology, pathogenesis, and biofilm formation ofMicrobiology and molecular biology reviews : MMBR · 2025Review
- Glycerol metabolism contributes to competition by oral streptococci through production of hydrogen peroxide.Journal of bacteriology · 2024Article
- Colonization of vancomycin-resistantFEMS microbes · 2024Article
- Enterococcal-host interactions in the gastrointestinal tract and beyond.FEMS microbes · 2024Review
- Identification of Multiple Iron Uptake Mechanisms in Enterococcus faecalis and Their Relationship to Virulence.Infection and immunity · 2023Article
- Associations of the skin, oral and gut microbiome with aging, frailty and infection risk reservoirs in older adults.Nature aging · 2022Article
- The NagY regulator: A member of the BglG/SacY antiterminator family conserved inFrontiers in microbiology · 2022Article
- Analysis of In Vivo Transcriptome of Intracellular Bacterial PathogenPathogens (Basel, Switzerland) · 2021Article
- Enterococcus faecalis Maltodextrin Gene Regulation by Combined Action of Maltose Gene Regulator MalR and Pleiotropic Regulator CcpA.Applied and environmental microbiology · 2020Article
- Study of key RNA metabolism proteins inRNA biology · 2020Article
- Fighting Pathogenic Bacteria on Two Fronts: Phages and Antibiotics as Combined Strategy.Frontiers in cellular and infection microbiology · 2019Review
- Complex Bacterial Consortia Reprogram the Colitogenic Activity ofFrontiers in immunology · 2019Article
- Manganese acquisition is essential for virulence of Enterococcus faecalis.PLoS pathogens · 2018Article
- Expression profiling in a mammalian host reveals the strong induction of genes encoding LysM domain-containing proteins in Enterococcus faecium.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterococcus faecalis is a Gram-positive lactic acid intestinal opportunistic bacterium with virulence potential. For a better understanding of the adapation of this bacterium to the host conditions, we performed a transcriptome analysis of bacteria isolated from an infection site (mouse peritonitis) by RNA-sequencing. We identified a total of 211 genes with significantly higher transcript levels and 157 repressed genes. Our in vivo gene expression database reflects well the infection process since genes encoding important virulence factors like cytolysin, gelatinase or aggregation substance as well as stress response proteins, are significantly induced. Genes encoding metabolic activities are the second most abundant in vivo induced genes demonstrating that the bacteria are metabolically active and adapt to the special nutrient conditions of the host. α- and β- glucosides seem to be important substrates for E. faecalis inside the host. Compared to laboratory conditions, the flux through the upper part of glycolysis seems to be reduced and more carbon may enter the pentose phosphate pathway. This may reflect the need of the bacteria under infection conditions to produce more reducing power for biosynthesis. Another important substrate is certainly glycerol since both pathways of glycerol catabolism are strongly induced. Strongly in vivo induced genes should be important for the infection process. This assumption has been verified in a virulence test using well characterized mutants affected in glycerol metabolism. This showed indeed that mutants unable to metabolize this sugar alcohol are affected in organ colonisation in a mouse model.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.