ArticleBiosensors & bioelectronics2015
A novel dynamic flow immunochromatographic test (DFICT) using gold nanoparticles for the serological detection of Toxoplasma gondii infection in dogs and cats.
Article in Biosensors & bioelectronics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 38 citations in OpenAlex.
- Rapid serological detection of smoothFrontiers in cellular and infection microbiology · 2026Article
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- A Novel Immunochromatographic Test Strip Using Lanthanide-Labeled Fluorescent Nanoparticles for the Serological Detection ofPathogens (Basel, Switzerland) · 2024Article
- Metallic Nanoparticles and Core-Shell Nanosystems in the Treatment, Diagnosis, and Prevention of Parasitic Diseases.Pathogens (Basel, Switzerland) · 2023Review
- Feline Toxoplasmosis in Greece: A Countrywide Seroprevalence Study and Associated Risk Factors.Pathogens (Basel, Switzerland) · 2022Article
- Biosensor Based Immunoassay: A New Approach for Serotyping ofNanomaterials (Basel, Switzerland) · 2021Article
- Prevalence of Toxoplasma gondii Antibodies in Stray Dogs from Various Locations in West and East Malaysia.The Korean journal of parasitology · 2020Article
- Perspectives of characterization and bioconjugation of gold nanoparticles and their application in lateral flow immunosensing.Drug delivery and translational research · 2020Review
- Review
- Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Review
- Study on Circulating Antigens in Serum of Mice With Experimental Acute Toxoplasmosis.Frontiers in microbiology · 2020Article
- Theranostics Aspects of Various Nanoparticles in Veterinary Medicine.International journal of molecular sciences · 2018Review
- Nanomedicine advances in toxoplasmosis: diagnostic, treatment, and vaccine applications.Parasitology research · 2017Review
- Sixty Years (1957-2017) of Research on Toxoplasmosis in China-An Overview.Frontiers in microbiology · 2017Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel dynamic flow immunochromatographic test (DFICT) is proposed for rapid assay utilizing Toxoplasma gondii as a model. The test is based on a proprietary technology that combines the principles of immunochromatography and fluid dynamics. Gold nanoparticles conjugated to staphylococcal protein A (SPA) were prepared in liquid form and used as signal vehicles. T. gondii-specific recombinant antigens and SPA were sprayed onto a nitrocellulose membrane in strips at positions designated as T and C, respectively. The DFICT is performed by applying a 100 µL aliquot of liquid gold-SPA conjugate to the reagent hole and a 5 μL aliquot of serum sample to the sample hole. The results were observable within 5 min by the naked eye. The lowest detectable limit of the assay was determined as the highest dilution (1:320) of positive serum. No cross-reaction of the antibodies with other related canine or feline pathogens was observed. The DFICT can be stored for 12 months at 4°C or 6 months with no loss of sensitivity or specificity. A high degree of consistency was observed between the DFICT and the standard ELISA kit, supporting the reliability of the novel test strip. The introduction of a liquid gold nanoparticle conjugate reagent provides this method with several attractive characteristics, such as ease of manufacture, low sample volume requirements, high selectivity and high efficiency. This method opens a novel pathway for rapid diagnostic screening and field analysis.
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