ArticlePloS one2015
A Drosophila Reporter for the Translational Activation of ATF4 Marks Stressed Cells during Development.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 56 citations in OpenAlex.
- Article
- The regulation of Xrp1 expression by uORFs and main ORF sequences and its function in Drosophila disease models.PLoS genetics · 2026Article
- Ring canals in the larval adipose of Drosophila buffer stress response.The Journal of cell biology · 2026Article
- Imaginal discs produce neuropeptide-like precursor 1 to alleviate ER stress in response to warm temperatures.Communications biology · 2025Article
- Emerging roles for integrated stress response signaling in homeostasis.The FEBS journal · 2025Review
- Loss of Paip1 causes translation reduction and induces apoptotic cell death through ISR activation and Xrp1.Cell death discovery · 2023Article
- Wg/Wnt1 and Erasp link ER stress to proapoptotic signaling in an autosomal dominant retinitis pigmentosa model.Experimental & molecular medicine · 2023Article
- Allnighter pseudokinase-mediated feedback links proteostasis and sleep in Drosophila.Nature communications · 2023Article
- The new uORFdb: integrating literature, sequence, and variation data in a central hub for uORF research.Nucleic acids research · 2023Article
- RACK1 is evolutionary conserved in satellite stem cell activation and adult skeletal muscle regeneration.Cell death discovery · 2022Article
- New Drosophila models to uncover the intrinsic and extrinsic factors that mediate the toxicity of the human prion protein.Disease models & mechanisms · 2022Article
- A protein-trap allele reveals roles for Drosophila ATF4 in photoreceptor degeneration, oogenesis and wing development.Disease models & mechanisms · 2022Article
- Pharmacological targeting of endoplasmic reticulum stress in disease.Nature reviews. Drug discovery · 2022Review
- Drosophila Unfolded Protein Response (UPR) Assays In Vitro and In Vivo.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Xrp1 and Irbp18 trigger a feed-forward loop of proteotoxic stress to induce the loser status.PLoS genetics · 2021Article
- The transcription factor Xrp1 is required for PERK-mediated antioxidant gene induction ineLife · 2021Article
- Translational induction of ATF4 during integrated stress response requires noncanonical initiation factors eIF2D and DENR.Nature communications · 2020Article
- Article
- Establishment and validation of an endoplasmic reticulum stress reporter to monitor zebrafish ATF6 activity in development and disease.Disease models & mechanisms · 2020Article
- The endoplasmic reticulum unfolded protein response varies depending on the affected region of the tissue but independently from the source of stress.Cell stress & chaperones · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Eukaryotic cells have evolved signaling pathways that help to restore cellular homeostasis in response to various physiological or pathological conditions. ATF4 is a transcription factor whose mRNA translation is stimulated in response to stress-activated eIF2alpha kinases. Established conditions that activate eIF2alpha phosphorylation and ATF4 translation include excessive stress in the endoplasmic reticulum (ER) and amino acid deprivation. ATF4 is activated through a unique translational activation mechanism that involves multiple upstream open reading frames (uORFs) in the 5'-untranslated region (UTR), which is conserved from yeast to mammals. Taking advantage of this, we developed a translational activation reporter of ATF4 in Drosophila, in which the dsRed reporter coding sequence was placed downstream of the Drosophila ATF4 5' UTR. This reporter remained inactive in most tissues under normal conditions, but showed dsRed expression when starved, or when challenged with conditions that imposed ER stress. In normally developing flies, a small number of cell types showed reporter expression even without exogenous stress, which included the salivary gland, gut, the male reproductive organ, and the photoreceptor cells, suggestive of inherent stress during the normal development of these cell types. These results establish a new tool to study ATF4-mediated stress response in Drosophila development and disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.