ArticlePloS one2015
Cardiac development in zebrafish and human embryonic stem cells is inhibited by exposure to tobacco cigarettes and e-cigarettes.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
65 citing papers in PubMed, 116 citations in OpenAlex.
- The XenCart Protocol: A method for Alcian blue labeling and quantitative analysis of craniofacial cartilage in Xenopus.Journal of anatomy · 2026Article
- Human Primary Mesenchymal Stem Cells Fail to Recover Full Osteogenic Differentiation Potential Following Washout of Electronic Cigarette Aerosol Extracts.Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco · 2026Article
- Prenatal Cannabis and Tobacco: Studies in Animal Models.Advances in experimental medicine and biology · 2026Review
- E-Cigarette and Vanillin Exposure Disrupts Cardiovascular Development in Xenopus laevis.Birth defects research · 2025Article
- The Impact of Maternal/In Utero Exposure of Novel Tobacco Products on the Offspring Cardiovascular Health.Cardiovascular toxicology · 2025Review
- Association of secondhand smoke exposure during pregnancy with left ventricle structure and function in offspring at 4 years old: a prospective birth cohort study.BMC pregnancy and childbirth · 2025Article
- A Novel Disulfidptosis-Related Diagnostic Gene Signature and Differential Expression Validation in Ischaemic Cardiomyopathy.Journal of cellular and molecular medicine · 2025Article
- Standardization and optimization of the hiPSC-based PluriLum assay for detection of embryonic and developmental toxicants.Archives of toxicology · 2024Article
- Multidisciplinary approaches in electronic nicotine delivery systems pulmonary toxicology: emergence of living and non-living bioinspired engineered systems.Communications engineering · 2024Review
- Addressing Cardiovascular Toxicity Risk of Electronic Nicotine Delivery Systems in the Twenty-First Century: "What Are the Tools Needed for the Job?" and "Do We Have Them?"Cardiovascular toxicology · 2024Review
- Investigating the applicability domain of the hiPSC-based PluriLum assay: an embryotoxicity assessment of chemicals and drugs.Archives of toxicology · 2024Article
- Zebrafish (International journal of molecular sciences · 2023Review
- Nicotine and Microvascular Responses in Skeletal Muscle from Acute Exposure to Cigarettes and Vaping.International journal of molecular sciences · 2023Article
- Electronic Nicotine Delivery Systems and Cardiovascular/Cardiometabolic Health.Circulation research · 2023Review
- Article
- A low-cost smartphone fluorescence microscope for research, life science education, and STEM outreach.Scientific reports · 2023Article
- Impact of E-cig aerosol vaping on fetal and neonatal respiratory development and function.Translational research : the journal of laboratory and clinical medicine · 2022Article
- Short-term effects of electronic cigarettes on cerebrovascular function: A time course study.Experimental physiology · 2022Article
- An assessment of vaping-induced inflammation and toxicity: A feasibility study using a 2-stage zebrafish and mouse platform.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2022Article
- Chronic Cardiotoxicity Assays Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPSC-CMs).International journal of molecular sciences · 2022Review
5 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMaternal smoking is a risk factor for low birth weight and other adverse developmental outcomes.
objectiveWe sought to determine the impact of standard tobacco cigarettes and e-cigarettes on heart development in vitro and in vivo.
methodsZebrafish (Danio rerio) were used to assess developmental effects in vivo and cardiac differentiation of human embryonic stem cells (hESCs) was used as a model for in vitro cardiac development.
resultsIn zebrafish, exposure to both types of cigarettes results in broad, dose-dependent developmental defects coupled with severe heart malformation, pericardial edema and reduced heart function. Tobacco cigarettes are more toxic than e-cigarettes at comparable nicotine concentrations. During cardiac differentiation of hESCs, tobacco smoke exposure results in a delayed transition through mesoderm. Both types of cigarettes decrease expression of cardiac transcription factors in cardiac progenitor cells, suggesting a persistent delay in differentiation. In definitive human cardiomyocytes, both e-cigarette- and tobacco cigarette-treated samples showed reduced expression of sarcomeric genes such as MLC2v and MYL6. Furthermore, tobacco cigarette-treated samples had delayed onset of beating and showed low levels and aberrant localization of N-cadherin, reduced myofilament content with significantly reduced sarcomere length, and increased expression of the immature cardiac marker smooth muscle alpha-actin.
conclusionThese data indicate a negative effect of both tobacco cigarettes and e-cigarettes on heart development in vitro and in vivo. Tobacco cigarettes are more toxic than E-cigarettes and exhibit a broader spectrum of cardiac developmental defects.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.