ArticleJournal of the Royal Society, Interface2015
A novel collagen gel-based measurement technique for quantitation of cell contraction force.
Article in Journal of the Royal Society, Interface, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Myocardial mechanics displaying on visual structural color hydrogel microcolumn arrays.Journal of nanobiotechnology · 2026Article
- Cell Contractile Force-Mediated Morphogenetic Tissue Engineering via 4D Printed Degradable Hydrogel Scaffolds.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Distinct Transcriptomic Profiles of Cultured Anterior and Posterior Populations of Human Infant Scleral Fibroblasts: Including Dopamine Receptors.Investigative ophthalmology & visual science · 2025Article
- Establishment of a tissue-engineered colon cancer model for comparative analysis of cancer cell lines.Journal of biomedical materials research. Part A · 2024Article
- Engineered colorectal cancer tissue recapitulates key attributes of a patient-derived xenograft tumor line.Biofabrication · 2022Article
- Soft Polymer-Based Technique for Cellular Force Sensing.Polymers · 2021Review
- AnMetabolites · 2021Article
- Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells.Polymers · 2021Article
- Mechanical homeostasis in tissue equivalents: a review.Biomechanics and modeling in mechanobiology · 2021Review
- Human pluripotent stem cell-derived cardiac stromal cells and their applications in regenerative medicine.Stem cell research · 2020Review
- Vascular Adhesion Protein-1 Determines the Cellular Properties of Endometrial Pericytes.Frontiers in cell and developmental biology · 2020Article
- Predictable fibroblast tension generation by measuring compaction of anchored collagen matrices using microscopy and optical coherence tomography.Cell adhesion & migration · 2019Article
- Biofabrication of phenotypic pulmonary fibrosis assays.Biofabrication · 2019Review
- Investigating Fibroblast-Induced Collagen Gel Contraction Using a Dynamic Microscale Platform.Frontiers in bioengineering and biotechnology · 2019Article
- Spatial distributions of pericellular stiffness in natural extracellular matrices are dependent on cell-mediated proteolysis and contractility.Acta biomaterialia · 2017Article
- Integration of growth factor gene delivery with collagen-triggered wound repair cascades using collagen-mimetic peptides.Bioengineering & translational medicine · 2016Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Cell contraction force plays an important role in wound healing, inflammation,angiogenesis and metastasis. This study describes a novel method to quantify single cell contraction force in vitro using human aortic adventitial fibroblasts embedded in a collagen gel. The technique is based on a depth sensing nano-indentation tester to measure the thickness and elasticity of collagen gels containing stimulated fibroblasts and a microscopy imaging system to estimate the gel area. In parallel, a simple theoretical model has been developed to calculate cell contraction force based on the measured parameters. Histamine (100 mM) was used to stimulate fibroblast contraction while the myosin light chain kinase inhibitor ML-7 (25 mM) was used to inhibit cell contraction. The collagen matrix used in the model provides a physiological environment for fibroblast contraction studies. Measurement of changes in collagen gel elasticity and thickness arising from histamine treatments provides a novel convenient technique to measure cell contraction force within a collagen matrix. This study demonstrates that histamine can elicit a significant increase in contraction force of fibroblasts embedded in collagen,while the Young's modulus of the gel decreases due to the gel degradation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.