ArticleNanoscale research letters2015
BMP2 gene delivery to bone mesenchymal stem cell by chitosan-g-PEI nonviral vector.
Article in Nanoscale research letters, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- Delamination of Multilayer Plastic Films to Recover Polyethylene with Favorable Mechanical Properties.ACS sustainable chemistry & engineering · 2026Article
- Polyethylenimine Carriers for Drug and Gene Delivery.Polymers · 2025Review
- Improvement of Biocatalytic Properties and Cytotoxic Activity of L-Asparaginase fromPharmaceuticals (Basel, Switzerland) · 2022Article
- Engineering stem cells to produce exosomes with enhanced bone regeneration effects: an alternative strategy for gene therapy.Journal of nanobiotechnology · 2022Article
- Development of novel gene carrier using modified nano hydroxyapatite derived from equine bone for osteogenic differentiation of dental pulp stem cells.Bioactive materials · 2021Article
- Folic Acid/Peptides Modified PLGA-PEI-PEG Polymeric Vectors as Efficient Gene Delivery Vehicles: Synthesis, Characterization and Their Biological Performance.Molecular biotechnology · 2021Article
- SP94-Targeted Triblock Copolymer Nanoparticle Delivers Thymidine Kinase-p53-Nitroreductase Triple Therapeutic Gene and Restores Anticancer Function against Hepatocellular Carcinoma in Vivo.ACS applied materials & interfaces · 2020Article
- Article
- Therapeutic effects of a mesenchymal stem cell‑based insulin‑like growth factor‑1/enhanced green fluorescent protein dual gene sorting system in a myocardial infarction rat model.Molecular medicine reports · 2018Article
- Polyethylenimine-based nanocarriers in co-delivery of drug and gene: a developing horizon.Nano reviews & experiments · 2018Review
- Efficient delivery of C/EBP beta gene into human mesenchymal stem cells via polyethylenimine-coated gold nanoparticles enhances adipogenic differentiation.Scientific reports · 2016Article
- Hyaluronic acid reagent functional chitosan-PEI conjugate with AQP2-siRNA suppressed endometriotic lesion formation.International journal of nanomedicine · 2016Article
- Nanotechnology for Stimulating Osteoprogenitor Differentiation.The open orthopaedics journal · 2016Article
- A branched TAT cell-penetrating peptide as a novel delivery carrier for the efficient gene transfection.Biomaterials research · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nanotechnology has made a significant impact on the development of nanomedicine. Nonviral vectors have been attracting more attention for the advantage of biosafety in gene delivery. Polyethylenimine (PEI)-conjugated chitosan (chitosan-g-PEI) emerged as a promising nonviral vector and has been demonstrated in many tumor cells. However, there is a lack of study focused on the behavior of this vector in stem cells which hold great potential in regenerative medicine. Therefore, in this study, in vitro gene delivering effect of chitosan-g-PEI was investigated in bone marrow stem cells. pIRES2-ZsGreen1-hBMP2 dual expression plasmid containing both the ZsGreen1 GFP reporter gene and the BMP2 functional gene was constructed for monitoring the transgene expression level. Chitosan-g-PEI-mediated gene transfer showed 17.2% of transfection efficiency and more than 80% of cell viability in stem cells. These values were higher than that of PEI. The expression of the delivered BMP2 gene in stem cells enhanced the osteogenic differentiation. These results demonstrated that chitosan-g-PEI is capable of applying in delivering gene to stem cells and providing potential applications in stem cell-based gene therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.