Evidence map›Paper›PMID 25977256›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2015

Angiopoietin-4 increases permeability of blood vessels and promotes lymphatic dilation.

Cristina T Kesler, Ethel R Pereira, Cheryl H Cui, Gregory M Nelson, David J Masuck, James W Baish, Timothy P Padera

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
1.1field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.

  1. Dynamic Remodeling of Oocyte-Granulosa Cell Communication During Bovine Folliculogenesis Revealed by Transcriptomic Meta-Analyses.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026
    Pooled it
  2. Trial
  3. Review
  4. Article
  5. Review
  6. Article
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  8. Article
  9. Article
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  11. Article
  12. Gene expression in retinal ischemic post-conditioning.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2018
    Article
  13. The Lymphatic System in Disease Processes and Cancer Progression.Annual review of biomedical engineering · 2016
    Review
  14. Measuring Vascular Permeability In Vivo.Methods in molecular biology (Clifton, N.J.) · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Cristina T Kesler*Edwin Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital, Boston, Massachusetts, USA; Harold B. Lee Library, Brigham Young University, Provo, Utah, USA; and Department of Biomedical Engineering, Bucknell University, Lewisburg, Pennsylvania, USA.
Ethel R Pereira*Edwin Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital, Boston, Massachusetts, USA; Harold B. Lee Library, Brigham Young University, Provo, Utah, USA; and Department of Biomedical Engineering, Bucknell University, Lewisburg, Pennsylvania, USA.
Cheryl H Cui*Edwin Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital, Boston, Massachusetts, USA; Harold B. Lee Library, Brigham Young University, Provo, Utah, USA; and Department of Biomedical Engineering, Bucknell University, Lewisburg, Pennsylvania, USA.
Gregory M Nelson*Edwin Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital, Boston, Massachusetts, USA; Harold B. Lee Library, Brigham Young University, Provo, Utah, USA; and Department of Biomedical Engineering, Bucknell University, Lewisburg, Pennsylvania, USA.
David J Masuck*Edwin Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital, Boston, Massachusetts, USA; Harold B. Lee Library, Brigham Young University, Provo, Utah, USA; and Department of Biomedical Engineering, Bucknell University, Lewisburg, Pennsylvania, USA.
James W Baish*Edwin Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital, Boston, Massachusetts, USA; Harold B. Lee Library, Brigham Young University, Provo, Utah, USA; and Department of Biomedical Engineering, Bucknell University, Lewisburg, Pennsylvania, USA.
Timothy P Padera*Edwin Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital, Boston, Massachusetts, USA; Harold B. Lee Library, Brigham Young University, Provo, Utah, USA; and Department of Biomedical Engineering, Bucknell University, Lewisburg, Pennsylvania, USA tpadera@steele.mgh.harvard.edu.
Brigham Young University · US

Funding

Characterizing lymphatic micrometastases: prognostic and therapeutic implicationsDP2OD008780 · OD · MASSACHUSETTS GENERAL HOSPITAL · PI PADERA, TIMOTHY P · 2011 to 2011
$2.6M
Lymphatic RadiobiologyR00CA137167 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI PADERA, TIMOTHY P · 2011 to 2013
$699k
PROTON THERAPY RESEARCH AND TREATMENT CENTERC06CA059267 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI GOITEIN, MICHAEL · 1992 to 1995
–
NCI NIH HHS C06 CA059267NCI NIH HHS R00 CA137167NCI NIH HHS R00CA137167NIH HHS DP2 OD008780NIH HHS DP2OD008780
6 · The paper itself

Abstract

The angiopoietin (Ang) ligands are potential therapeutic targets for lymphatic related diseases, which include lymphedema and cancer. Ang-1 and Ang-2 functions are established, but those of Ang-4 are poorly understood. We used intravital fluorescence microscopy to characterize Ang-4 actions on T241 murine fibrosarcoma-associated vessels in mice. The diameters of lymphatic vessels draining Ang-4- or VEGF-C (positive control)-expressing tumors increased to 123 and 135 μm, respectively, and parental, mock-transduced (negative controls) and tumors expressing Ang-1 or Ang-2 remained at baseline (∼60 μm). Ang-4 decreased human dermal lymphatic endothelial cell (LEC) monolayer permeability by 27% while increasing human dermal blood endothelial cell (BEC) monolayer permeability by 200%. In vivo, Ang-4 stimulated a 4.5-fold increase in tumor-associated blood vessel permeability compared with control when measured using intravital quantitative multiphoton microscopy. Ang-4 activated receptor signaling in both LECs and BECs, evidenced by tyrosine kinase with Ig and endothelial growth factor homology domains-2 (TIE2) receptor, protein kinase B, and Erk1,2 phosphorylation detectable by immunoblotting. These data suggest that Ang-4 actions are mediated through cell-type-specific networks and that lymphatic vessel dilation occurs secondarily to increased vascular leakage. Ang-4 also promoted survival of LECs. Thus, blocking Ang-4 may prune the draining lymphatic vasculature and decrease interstitial fluid pressure (IFP) by reducing vascular permeability.

Indexed as

Capillary PermeabilityAngiopoietinsAnimalsEndothelial CellsFibrosarcomaHumansLymphatic VesselsMiceMice, NudeMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Neoplasms, ExperimentalNeovascularization, PathologicVascular Endothelial Growth Factor Cangiopoietin 4AngiopoietinsMAPK1 protein, humanMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Vascular Endothelial Growth Factor Cvascular endothelial growth factor C, mouseVEGFC protein, human2-photon microscopyendotheliallymphangiographyTIE2

Identifiers

PMID25977256
PMCPMC4550378
OpenAlexW2105925331

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.