ArticleScientific reports2015
Real-time monitoring of cell protrusion dynamics by impedance responses.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
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- Ras-mediated dynamic and biphasic regulation of cell migration.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- p16Immunity & ageing : I & A · 2024Article
- Optogenetically engineered Septin-7 enhances immune cell infiltration of tumor spheroids.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- MLL1 regulates cytokine-driven cell migration and metastasis.Science advances · 2024Article
- Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles.Nature communications · 2023Article
- Differential response of MDA‑MB‑231 breast cancer and MCF10A normal breast cells to cytoskeletal disruption.Oncology reports · 2023Article
- Ion Channels in Gliomas-From Molecular Basis to Treatment.International journal of molecular sciences · 2023Review
- Quantifying protrusions as tumor-specific biophysical predictors of cancer invasion in in vitro tumor micro-spheroid models.In vitro models · 2022Article
- Chronic arsenic increases cell migration in BEAS-2B cells by increasing cell speed, cell persistence, and cell protrusion length.Experimental cell research · 2021Article
- Article
- PLEKHG5 regulates autophagy, survival and MGMT expression in U251-MG glioblastoma cells.Scientific reports · 2020Article
- RSU-1 Maintains Integrity ofG3 (Bethesda, Md.) · 2020Article
- Bio-impedance measurement allows displaying the early stages of neutrophil extracellular traps.EXCLI journal · 2020Article
- Microstencil-based spatial immobilization of individual cells for single cell analysis.Biomicrofluidics · 2018Article
- A new temperature-dependent strategy to modulate the epidermal growth factor receptor.Biomaterials · 2018Article
- Inhibition of ovarian tumor cell invasiveness by targeting SYK in the tyrosine kinase signaling pathway.Oncogene · 2018Article
- Numerical investigation of the role of intercellular interactions on collective epithelial cell migration.Biomechanics and modeling in mechanobiology · 2018Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellular protrusions are highly dynamic structures involved in fundamental processes, including cell migration and invasion. For a cell to migrate, its leading edge must form protrusions, and then adhere or retract. The spatial and temporal coordination of protrusions and retraction is yet to be fully understood. The study of protrusion dynamics mainly relies on live-microscopy often coupled to fluorescent labeling. Here we report the use of an alternative, label-free, quantitative and rapid assay to analyze protrusion dynamics in a cell population based on the real-time recording of cell activity by means of electronic sensors. Cells are seeded on a plate covered with electrodes and their shape changes map into measured impedance variations. Upon growth factor stimulation the impedance increases due to protrusive activity and decreases following retraction. Compared to microscopy-based methods, impedance measurements are suitable to high-throughput studies on different cell lines, growth factors and chemical compounds. We present data indicating that this assay lends itself to dissect the biochemical signaling pathways controlling adhesive protrusions. Indeed, we show that the protrusion phase is sustained by actin polymerization, directly driven by growth factor stimulation. Contraction instead mainly relies on myosin action, pointing at a pivotal role of myosin in lamellipodia retraction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.