ArticleBMC plant biology2015
Enhanced somatic embryogenesis in Theobroma cacao using the homologous BABY BOOM transcription factor.
Article in BMC plant biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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72 citing papers in PubMed, 186 citations in OpenAlex.
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- Stage-specific metabolic divergence in flavonoid biosynthesis correlates with embryogenic capacity in rubber tree (Frontiers in plant science · 2026Article
- Woody Plant Transformation: Current Status, Challenges, and Future Perspectives.Plants (Basel, Switzerland) · 2025Review
- CRISPR/Cas9-mediated gene targeting at BBM2 locus demonstrates HDR-assisted precise knock-in in banana cv. Grand Naine.Plant cell reports · 2025Article
- Morphogenetic Factors as a Tool for Enhancing Plant Regeneration Capacity During In Vitro Transformation.International journal of molecular sciences · 2025Review
- Current Advancement and Future Prospects in Simplified Transformation-Based Plant Genome Editing.Plants (Basel, Switzerland) · 2025Review
- PacBio single-molecule long-read transcriptome sequencing and analysis of somatic embryogenesis inFrontiers in plant science · 2025Article
- Genome-Wide Identification and Expression Analysis of theInternational journal of molecular sciences · 2024Article
- Functional Mechanisms and the Application of Developmental Regulators for Improving Genetic Transformation in Plants.Plants (Basel, Switzerland) · 2024Review
- Appreciating animal induced pluripotent stem cells to shape plant cell reprogramming strategies.Journal of experimental botany · 2024Review
- Enhancing wheat regeneration and genetic transformation through overexpression of TaLAX1.Plant communications · 2024Article
- Application of Developmental Regulators for Enhancing Plant Regeneration and Genetic Transformation.Plants (Basel, Switzerland) · 2024Review
- Integrated metabolomics and transcriptomics analysis highlight key pathways involved in the somatic embryogenesis of Darjeeling tea.BMC genomics · 2024Article
- The improvement of the in vitro plant regeneration in barley with the epigenetic modifier of histone acetylation, trichostatin A.Journal of applied genetics · 2024Article
- Promoting genotype-independent plant transformation by manipulating developmental regulatory genes and/or using nanoparticles.Plant cell reports · 2023Review
- Advances in Somatic Embryogenesis of Banana.International journal of molecular sciences · 2023Review
- Genome-Wide Identification of Homeodomain Leucine Zipper (HD-ZIP) Transcription Factor, Expression Analysis, and Protein Interaction of HD-ZIP IV in Oil Palm Somatic Embryogenesis.International journal of molecular sciences · 2023Article
- Global transcriptome profiling reveals differential regulatory, metabolic and hormonal networks during somatic embryogenesis in Coffea arabica.BMC genomics · 2023Article
12 more citing papers are in PubMed but not listed here.
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5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTheobroma cacao, the chocolate tree, is an important economic crop in East Africa, South East Asia, and South and Central America. Propagation of elite varieties has been achieved through somatic embryogenesis (SE) but low efficiencies and genotype dependence still presents a significant limitation for its propagation at commercial scales. Manipulation of transcription factors has been used to enhance the formation of SEs in several other plant species. This work describes the use of the transcription factor Baby Boom (BBM) to promote the transition of somatic cacao cells from the vegetative to embryonic state.
resultsAn ortholog of the Arabidopsis thaliana BBM gene (AtBBM) was characterized in T. cacao (TcBBM). TcBBM expression was observed throughout embryo development and was expressed at higher levels during SE as compared to zygotic embryogenesis (ZE). TcBBM overexpression in A. thaliana and T. cacao led to phenotypes associated with SE that did not require exogenous hormones. While transient ectopic expression of TcBBM provided only moderate enhancements in embryogenic potential, constitutive overexpression dramatically increased SE proliferation but also appeared to inhibit subsequent development.
conclusionOur work provides validation that TcBBM is an ortholog to AtBBM and has a specific role in both somatic and zygotic embryogenesis. Furthermore, our studies revealed that TcBBM transcript levels could serve as a biomarker for embryogenesis in cacao tissue. Results from transient expression of TcBBM provide confirmation that transcription factors can be used to enhance SE without compromising plant development and avoiding GMO plant production. This strategy could compliment a hormone-based method of reprogramming somatic cells and lead to more precise manipulation of SE at the regulatory level of transcription factors. The technology would benefit the propagation of elite varieties with low regeneration potential as well as the production of transgenic plants, which similarly requires somatic cell reprogramming.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.