ArticleCell stress & chaperones2015
Caco-2 cells infected with rotavirus release extracellular vesicles that express markers of apoptotic bodies and exosomes.
Article in Cell stress & chaperones, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Extracellular vesicles in diagnostics and therapy of the ischaemic heart: Position Paper from the Working Group on Cellular Biology of the Heart of the European Society of Cardiology.Cardiovascular research · 2018Guideline
- Astroviruses use mucus secretion as a novel form of viral egress.PLoS pathogens · 2026Article
- Rethinking Histones as Negative Markers for the Characterization of Extracellular Vesicles.Journal of extracellular biology · 2026Article
- Characteristics of Exosomes and Microbiota in the Decidua of Patients With Early Pregnancy Loss and Their Impact on Trophoblast Cells.Molecular reproduction and development · 2026Article
- Experimental adaptation of pigeon rotavirus A (pRVA) in human colorectal cancer cells reveals interferon-driven host responses and immune checkpoint modulation.New microbes and new infections · 2026Article
- Isolation and whole-genome characterization of an emerging G9P[23] porcine rotavirus in Central China.Frontiers in veterinary science · 2025Article
- Exploring the exoproteome of the parasitic nematodeFrontiers in immunology · 2025Article
- Engineering strategies for apoptotic bodies.Smart medicine · 2024Review
- Mammalian orthoreovirus can exit cells in extracellular vesicles.PLoS pathogens · 2024Article
- Nonenveloped Avian Reoviruses Released with Small Extracellular Vesicles Are Highly Infectious.Viruses · 2023Article
- Inflammatory Response in Caco-2 Cells Stimulated withPathogens (Basel, Switzerland) · 2022Article
- Protective effects of the postbiotic deriving from cow's milk fermentation with L. paracasei CBA L74 against Rotavirus infection in human enterocytes.Scientific reports · 2022Article
- Challenges and opportunities in the study of extracellular vesicles: Global institutional context and national state of the artBiomedica : revista del Instituto Nacional de Salud · 2021Article
- Egress of non-enveloped enteric RNA viruses.The Journal of general virology · 2021Review
- Assessment of Placental Extracellular Vesicles-Associated Fas Ligand and TNF-Related Apoptosis-Inducing Ligand in Pregnancies Complicated by Early and Late Onset Preeclampsia.Frontiers in physiology · 2021Article
- Assessing the oncolytic potential of rotavirus on mouse myeloma cell line Sp2/0-Ag14.Biomedica : revista del Instituto Nacional de Salud · 2020Article
- Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926.PloS one · 2020Article
- Erythroid Progenitor Cells in Atlantic Salmon (Viruses · 2019Article
- Advances in Diagnostic Approaches for Viral Etiologies of Diarrhea: From the Lab to the Field.Frontiers in microbiology · 2019Review
- Establishment of porcine enterocyte/myofibroblast co-cultures for the growth of porcine rota- and coronaviruses.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, we showed that infecting human intestinal epithelial cells (Caco-2) with rotavirus (RV) increases the release of extracellular vesicles (EVs) with an immunomodulatory function that, upon concentration at 100,000×g, present buoyant densities on a sucrose gradient of between 1.10 to 1.18 g/ml (characteristic of exosomes) and higher than 1.24 g/ml (proposed for apoptotic bodies). The effect of cellular death induced by RV on the composition of these EV is unknown. Here, we evaluated exosome (CD63, Hsc70, and AChE) and apoptotic body (histone H3) markers in EVs isolated by differential centrifugation (4000×g, 10,000×g, and 100,000×g) or filtration/ultracentrifugation (100,000×g) protocols. When we infected cells in the presence of caspase inhibitors, Hsc70 and AChE diminished in EVs obtained at 100,000×g, but not in EVs obtained at 4000×g or 10,000×g. In addition, caspase inhibitors decreased CD63 and AChE in vesicles with low and high buoyant densities. Without caspase inhibitors, RV infection increased exosome markers in all of the EVs obtained by differential centrifugation. However, CD63 preferentially localized in the 100,000×g fraction and H3 only increased in EVs concentrated at 100,000×g and with high buoyant densities on a sucrose gradient. Thus, RV infection increases the release of EVs that, upon concentration at 100,000×g, are composed by exosomes and apoptotic bodies, which can partially be separated using sucrose gradients.
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