ArticleRedox biology2015
Fluorescence labeling of carbonylated lipids and proteins in cells using coumarin-hydrazide.
Article in Redox biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Using Redox Proteomics to Gain New Insights into Neurodegenerative Disease and Protein Modification.Antioxidants (Basel, Switzerland) · 2024Review
- Spatial activity mapping of ß-mannanase on soybean seeds.Scientific reports · 2024Article
- Zika virus remodelled ER membranes contain proviral factors involved in redox and methylation pathways.Nature communications · 2023Article
- Hydropersulfides inhibit lipid peroxidation and ferroptosis by scavenging radicals.Nature chemical biology · 2023Article
- New Azido Coumarins as Potential Agents for Fluorescent Labeling and Their "Click" Chemistry Reactions for the Conjugation withMolecules (Basel, Switzerland) · 2022Article
- A Novel Fluorogenic Assay for the Detection of Nephrotoxin-Induced Oxidative Stress in Live Cells and Renal Tissue.ACS sensors · 2021Article
- Design of Fluorescent Probes for Bioorthogonal Labeling of Carbonylation in Live Cells.Scientific reports · 2020Article
- Impact of inhibition of the autophagy-lysosomal pathway on biomolecules carbonylation and proteome regulation in rat cardiac cells.Redox biology · 2019Article
- CytochromeThe Journal of biological chemistry · 2019Article
- Protein carbonyl determination by a rhodamine B hydrazide-based fluorometric assay.Redox biology · 2018Article
- Protein and cell wall polysaccharide carbonyl determination by a neutral pH 2,4-dinitrophenylhydrazine-based photometric assay.Redox biology · 2018Article
- Cross-talk between lipid and protein carbonylation in a dynamic cardiomyocyte model of mild nitroxidative stress.Redox biology · 2017Article
- Benzocoumarin Hydrazine: A Large Stokes Shift Fluorogenic Sensor for Detecting Carbonyls in Isolated Biomolecules and in Live Cells.ACS sensors · 2017Article
- A biphasic effect of TNF-α in regulation of the Keap1/Nrf2 pathway in cardiomyocytes.Redox biology · 2016Article
- Structural, biological and biophysical properties of glycated and glycoxidized phosphatidylethanolamines.Free radical biology & medicine · 2016Article
- Antibiotic Susceptibility Testing of the Gram-Negative Bacteria Based on Flow Cytometry.Frontiers in microbiology · 2016Article
- Current Probes for Imaging Carbonylation in Cellular Systems and Their Relevance to Progression of Diseases.Technology in cancer research & treatmentReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carbonylation is a generic term which refers to reactive carbonyl groups present in biomolecules due to oxidative reactions induced by reactive oxygen species. Carbonylated proteins, lipids and nucleic acids have been intensively studied and often associated with onset or progression of oxidative stress related disorders. In order to reveal underlying carbonylation pathways and biological relevance, it is crucial to study their intracellular formation and spatial distribution. Carbonylated species are usually identified and quantified in cell lysates and body fluids after derivatization using specific chemical probes. However, spatial cellular and tissue distribution have been less often investigated. Here, we report coumarin-hydrazide, a fluorescent chemical probe for time- and cost-efficient labeling of cellular carbonyls followed by fluorescence microscopy to evaluate their intracellular formation both in time and space. The specificity of coumarin-hydrazide was confirmed in time- and dose-dependent experiments using human primary fibroblasts stressed with paraquat and compared with conventional DNPH-based immunocytochemistry. Both techniques stained carbonylated species accumulated in cytoplasm with strong perinuclear clustering. Using a complimentary array of analytical methods specificity of coumarin-hydrazide probe towards both protein- and lipid-bound carbonyls has been shown. Additionally, co-distribution of carbonylated species and oxidized phospholipids was demonstrated.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.