Evidence map›Paper›PMID 25974523›Full record

ArticlePhysical review. E, Statistical, nonlinear, and soft matter physics2015

Super-resolution two-photon microscopy via scanning patterned illumination.

Ben E Urban, Ji Yi, Siyu Chen, Biqin Dong, Yongling Zhu, Steven H DeVries, Vadim Backman, Hao F Zhang

Abstract read
In one paragraph

Article in Physical review. E, Statistical, nonlinear, and soft matter physics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Advances in Wearable Bioimaging.Advanced materials (Deerfield Beach, Fla.) · 2026
    Review
  2. Article
  3. Review
  4. iScience · 2022
    Review
  5. Article
  6. Article
  7. Recent advances in nonlinear microscopy: Deep insights and polarized revelations.The international journal of biochemistry & cell biology · 2021
    Review
  8. Brief update on endocytosis of nanomedicines.Advanced drug delivery reviews · 2019
    Review
  9. Article
  10. Article
  11. In Vivo Superresolution Imaging of Neuronal Structure in the Mouse Brain.IEEE transactions on bio-medical engineering · 2018
    Article
  12. Review
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ben E UrbanDepartment of Biomedical Engineering, Northwestern University, Evanston, Illinois, USA.
Ji YiDepartment of Biomedical Engineering, Northwestern University, Evanston, Illinois, USA.
Siyu ChenDepartment of Biomedical Engineering, Northwestern University, Evanston, Illinois, USA.
Biqin DongDepartment of Biomedical Engineering, Northwestern University, Evanston, Illinois, USA.
Yongling ZhuDepartment of Ophthalmology, Northwestern University, Chicago, Illinois, USA.
Steven H DeVriesDepartment of Ophthalmology, Northwestern University, Chicago, Illinois, USA.
Vadim BackmanDepartment of Biomedical Engineering, Northwestern University, Evanston, Illinois, USA.
Hao F ZhangDepartment of Biomedical Engineering, Northwestern University, Evanston, Illinois, USA.

Funding

Tumor Environment and Metastasis (TEAM) Research ProgramP30CA060553 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI Daniela E Matei · 1993 to 2026
$153.9M
Signal Processing in the Inner RetinaR01EY018204 · NEI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI DEVRIES, STEVEN H, ZHU, YONGLING · 2007 to 2024
$6.4M
Novel Antiangiogenic Peptides for Treatment of Exudative AMDR24EY022883 · NEI · UNIVERSITY OF WISCONSIN-MADISON · PI SHEIBANI, NADER · 2013 to 2017
$6.2M
Multimodal Retinal Functional Imaging for Diabetic RetinopathyR01EY019951 · NEI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI FAWZI, AMANI A, JIAO, SHULIANG · 2011 to 2015
$3.0M
Mechanisms of anterior segment development and corneal neovascularizationR01EY019484 · NEI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI KUME, TSUTOMU · 2010 to 2014
$1.5M
NCI NIH HHS P30 CA060553NEI NIH HHS R01 EY018204NEI NIH HHS R01EY018204NEI NIH HHS R01 EY019484NEI NIH HHS R01EY019484NEI NIH HHS R01 EY019951NEI NIH HHS R01EY019951NEI NIH HHS R24 EY022883NEI NIH HHS R24EY022883
6 · The paper itself

Abstract

We developed two-photon scanning patterned illumination microscopy (2P-SPIM) for super-resolution two-photon imaging. Our approach used a traditional two-photon microscopy setup with temporally modulated excitation to create patterned illumination fields. Combing nine different illuminations and structured illumination reconstruction, super-resolution imaging was achieved in two-photon microscopy. Using 2P-SPIM we achieved a lateral resolution of 141 nm, which represents an improvement by a factor of 1.9 over the corresponding diffraction limit. We further demonstrated super-resolution cellular imaging by 2P-SPIM to image actin cytoskeleton in mammalian cells and three-dimensional imaging in highly scattering retinal tissue.

Indexed as

Actin CytoskeletonContrast MediaEquipment DesignFluorescenceGreen Fluorescent ProteinsHeLa CellsHumansImaging, Three-DimensionalMicroscopy, FluorescenceModels, TheoreticalNanospheresPhantoms, ImagingQuinolinium CompoundsRetinaTissue Culture TechniquesAlexa fluor 546Contrast MediaGreen Fluorescent ProteinsQuinolinium Compounds

Identifiers

PMID25974523
PMCPMC4565794

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.