Evidence map›Paper›PMID 25974401›Full record

ArticleEndocrinology2015

Two Zebrafish hsd3b Genes Are Distinct in Function, Expression, and Evolution.

Jen-Chieh Lin, Shing Hu, Pei-Hung Ho, Hwei-Jan Hsu, John H Postlethwait, Bon-chu Chung

Erratum issuedOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.3field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 27 citations in OpenAlex.

  1. Knockout ofZoological research · 2026
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  4. Direct male development in chromosomally ZZ zebrafish.Frontiers in cell and developmental biology · 2024
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Jen-Chieh LinInstitute of Genome Sciences (J.-C.L., P.-H.H., B.-c.C.), National Yang-Ming University, Taipei, 112 Taiwan; Institute of Molecular Biology (J.-C.L., S.H., P.-H.H., H.-J.H., B.-c.C.), Academia Sinica, Taipei, 115 Taiwan; and Institute of Neuroscience (J.H.P.), University of Oregon, Eugene, Oregon 97403.
Shing HuInstitute of Genome Sciences (J.-C.L., P.-H.H., B.-c.C.), National Yang-Ming University, Taipei, 112 Taiwan; Institute of Molecular Biology (J.-C.L., S.H., P.-H.H., H.-J.H., B.-c.C.), Academia Sinica, Taipei, 115 Taiwan; and Institute of Neuroscience (J.H.P.), University of Oregon, Eugene, Oregon 97403.
Pei-Hung HoInstitute of Genome Sciences (J.-C.L., P.-H.H., B.-c.C.), National Yang-Ming University, Taipei, 112 Taiwan; Institute of Molecular Biology (J.-C.L., S.H., P.-H.H., H.-J.H., B.-c.C.), Academia Sinica, Taipei, 115 Taiwan; and Institute of Neuroscience (J.H.P.), University of Oregon, Eugene, Oregon 97403.
Hwei-Jan HsuInstitute of Genome Sciences (J.-C.L., P.-H.H., B.-c.C.), National Yang-Ming University, Taipei, 112 Taiwan; Institute of Molecular Biology (J.-C.L., S.H., P.-H.H., H.-J.H., B.-c.C.), Academia Sinica, Taipei, 115 Taiwan; and Institute of Neuroscience (J.H.P.), University of Oregon, Eugene, Oregon 97403.
John H PostlethwaitInstitute of Genome Sciences (J.-C.L., P.-H.H., B.-c.C.), National Yang-Ming University, Taipei, 112 Taiwan; Institute of Molecular Biology (J.-C.L., S.H., P.-H.H., H.-J.H., B.-c.C.), Academia Sinica, Taipei, 115 Taiwan; and Institute of Neuroscience (J.H.P.), University of Oregon, Eugene, Oregon 97403.
Bon-chu ChungInstitute of Genome Sciences (J.-C.L., P.-H.H., B.-c.C.), National Yang-Ming University, Taipei, 112 Taiwan; Institute of Molecular Biology (J.-C.L., S.H., P.-H.H., H.-J.H., B.-c.C.), Academia Sinica, Taipei, 115 Taiwan; and Institute of Neuroscience (J.H.P.), University of Oregon, Eugene, Oregon 97403.
Institute of Molecular Biology, Academia Sinica · TWNational Yang Ming Chiao Tung University · TWUniversity of Oregon · US

Funding

Resources for Teleost Gene Duplicates and Human DiseaseR01OD011116 · OD · UNIVERSITY OF OREGON · PI POSTLETHWAIT, JOHN H. · 2012 to 2020
$5.4M
Mechanisms of Sex Determination in ZebrafishR01GM085318 · NIGMS · UNIVERSITY OF OREGON · PI POSTLETHWAIT, JOHN H. · 2010 to 2018
$2.1M
NIGMS NIH HHS R01 GM085318NIH HHS R01 OD011116
6 · The paper itself

Abstract

HSD3B catalyzes the synthesis of δ4 steroids such as progesterone in the adrenals and gonads. Individuals lacking HSD3B2 activity experience congenital adrenal hyperplasia with imbalanced steroid synthesis. To develop a zebrafish model of HSD3B deficiency, we characterized 2 zebrafish hsd3b genes. Our phylogenetic and conserved synteny analyses showed that the tandemly duplicated human HSD3B1 and HSD3B2 genes are coorthologs of zebrafish hsd3b1 on chromosome 9 (Dre9), whereas the gene called hsd3b2 resides on Dre20 in an ancestral chromosome segment, from which its ortholog was lost in the tetrapod lineage. Zebrafish hsd3b1(Dre 9) was expressed in adult gonads and headkidney, which contains interrenal glands, the zebrafish counterpart of the tetrapod adrenal. Knockdown of hsd3b1(Dre 9) caused the interrenal and anterior pituitary to expand and pigmentation to increase, resembling human HSD3B2 deficiency. The zebrafish hsd3b2(Dre 20) gene was expressed in zebrafish early embryos as maternal transcripts that disappeared 1 day after fertilization. Morpholino inactivation of hsd3b2(Dre 20) led to embryo elongation, which was rescued by the injection of hsd3b2 mRNA. Thus, zebrafish hsd3b2(Dre 20) evolved independently of hsd3b1(Dre 9) with a morphogenetic function during early embryogenesis. Zebrafish hsd3b1(Dre 9), on the contrary, functions like mammalian HSD3B2, whose deficiency leads to congenital adrenal hyperplasia.

Indexed as

Evolution, MolecularAnimalsAnimals, Genetically ModifiedChlorocebus aethiopsCOS CellsEmbryo, NonmammalianGene Expression Regulation, EnzymologicGene Knockdown TechniquesMultienzyme ComplexesPhylogenyProgesterone ReductaseSteroid IsomerasesZebrafish3 beta-hydroxysteroid dehydrogenase type II3 beta-hydroxysteroid oxidoreductase-delta(5) 3-ketosteroid isomeraseMultienzyme ComplexesProgesterone ReductaseSteroid Isomerases

Identifiers

PMID25974401
PMCPMC4511139
OpenAlexW2189709647

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.