ArticleJournal of microscopy2015
Metallothionein as a clonable tag for protein localization by electron microscopy of cells.
Article in Journal of microscopy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- Labeling and Localization Strategies for In Situ Cryo-Electron Tomography Across the Viral Life Cycle.Viruses · 2026Review
- Cloneable contrast across all biological length scales.Journal of structural biology · 2026Article
- Genetically encoded barcodes for correlative volume electron microscopy.Nature biotechnology · 2023Article
- Cloneable inorganic nanoparticles.Chemical communications (Cambridge, England) · 2023Review
- Volume electron microscopy.Nature reviews. Methods primers · 2022Article
- DNA origami signposts for identifying proteins on cell membranes by electron cryotomography.Cell · 2021Article
- Genetically encoded tags for direct synthesis of EM-visible gold nanoparticles in cells.Nature methods · 2020Article
- Cryogenic single-molecule fluorescence annotations for electron tomography reveal in situ organization of key proteins inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Glucose starvation triggers filamentous septin assemblies in anBiology open · 2019Article
- The viral replication organelles within cells studied by electron microscopy.Advances in virus research · 2019Article
- VIPER is a genetically encoded peptide tag for fluorescence and electron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Locating macromolecules and determining structures inside bacterial cells using electron cryotomography.Biochimica et biophysica acta. Proteins and proteomics · 2018Review
- FerriTag is a new genetically-encoded inducible tag for correlative light-electron microscopy.Nature communications · 2018Article
- A TEM-traceable physiologically functional gold nanoprobe that permeates non-endocytic cells.International journal of nanomedicine · 2018Article
- Determining the target protein localization in 3D using the combination of FIB-SEM and APEX2.Biophysics reports · 2017Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
Abstract
A benign, clonable tag for the localization of proteins by electron microscopy of cells would be valuable, especially if it provided labelling with high signal-to-noise ratio and good spatial resolution. Here we explore the use of metallothionein as such a localization marker. We have achieved good success with desmin labelled in vitro and with a component of the yeast spindle pole body labelled in cells. Heavy metals added after fixation and embedding or during the process of freeze-substitution fixation provide readily visible signals with no concern that the heavy atoms are affecting the behaviour of the protein in its physiological environment. However, our methods did not work with protein components of the nuclear pore complex, suggesting that this approach is not yet universally applicable. We provide a full description of our optimal labelling conditions and other conditions tried, hoping that our work will allow others to label their own proteins of interest and/or improve on the methods we have defined.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.