Evidence map›Paper›PMID 25974161›Full record

ArticlePloS one2015

Diacylglycerol O-acyltransferase type-1 synthesizes retinyl esters in the retina and retinal pigment epithelium.

Joanna J Kaylor, Roxana A Radu, Nicholas Bischoff, Jacob Makshanoff, Jane Hu, Marcia Lloyd, Shannan Eddington, Tran Bianconi, Dean Bok, Gabriel H Travis

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Advances and therapeutic opportunities in visual cycle modulation.Progress in retinal and eye research · 2025
    Review
  3. Review
  4. Ablation of Fatty Acid Transport Protein-4 Enhances Cone Survival, M-cone Vision, and Synthesis of Cone-Tropic 9-The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024
    Article
  5. Review
  6. Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Pharmacological Amelioration of Cone Survival and Vision in a Mouse Model for Leber Congenital Amaurosis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Joanna J KaylorJules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America.
Roxana A RaduJules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America.
Nicholas BischoffJules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America.
Jacob MakshanoffJules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America.
Jane HuJules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America.
Marcia LloydJules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America.
Shannan EddingtonJules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America.
Tran BianconiJules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America.
Dean BokJules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America; Department of Neurobiology, University of California Los Angeles, Los Angeles, California, United States of America.
Gabriel H TravisJules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America; Department of Biological Chemistry, University of California Los Angeles, Los Angeles, California, United States of America.
University of California, Los Angeles · US

Funding

Vision Research Core at UCLAP30EY000331 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Gregory Darin Field, DAVID S WILLIAMS · 1985 to 2026
$16.8M
The Role of Muller Cells in Visual Pigment RegenerationR01EY011713 · NEI · UNIVERSITY OF TEXAS SW MED CTR/DALLAS · PI TRAVIS, GABRIEL H · 1997 to 2017
$6.9M
NEI NIH HHS P30 EY000331NEI NIH HHS P30-EY00331NEI NIH HHS R01 EY011713NEI NIH HHS R01-EY11713
6 · The paper itself

Abstract

Retinyl esters represent an insoluble storage form of vitamin A and are substrates for the retinoid isomerase (Rpe65) in cells of the retinal pigment epithelium (RPE). The major retinyl-ester synthase in RPE cells is lecithin:retinol acyl-transferase (LRAT). A second palmitoyl coenzyme A-dependent retinyl-ester synthase activity has been observed in RPE homogenates but the protein responsible has not been identified. Here we show that diacylglycerol O-acyltransferase-1 (DGAT1) is expressed in multiple cells of the retina including RPE and Müller glial cells. DGAT1 catalyzes the synthesis of retinyl esters from multiple retinol isomers with similar catalytic efficiencies. Loss of DGAT1 in dgat1(-/-) mice has no effect on retinal anatomy or the ultrastructure of photoreceptor outer-segments (OS) and RPE cells. Levels of visual chromophore in dgat1(-/-) mice were also normal. However, the normal build-up of all-trans-retinyl esters (all-trans-RE's) in the RPE during the first hour after a deep photobleach of visual pigments in the retina was not seen in dgat1(-/-) mice. Further, total retinyl-ester synthase activity was reduced in both dgat1(-/-) retina and RPE.

Indexed as

AcyltransferasesAnimalsCells, Culturedcis-trans-IsomerasesDiacylglycerol O-AcyltransferaseEstersGene DeletionMice, Inbred BALB CMice, Inbred C57BLPalmitoyl Coenzyme ARetinaRetinaldehydeRetinal Pigment EpitheliumRetinoid IsomerohydrolaseVitamin AAcyltransferasescis-trans-IsomerasesDiacylglycerol O-AcyltransferaseEsterslecithin-retinol acyltransferasePalmitoyl Coenzyme ARetinaldehydeRetinoid IsomerohydrolaseVitamin A

Identifiers

PMID25974161
PMCPMC4431840
OpenAlexW402970430

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.