Evidence map›Paper›PMID 25974096›Full record

ArticleNature protocols2015

Inexpensive, rapid prototyping of microfluidic devices using overhead transparencies and a laser print, cut and laminate fabrication method.

Brandon L Thompson, Yiwen Ouyang, Gabriela R M Duarte, Emanuel Carrilho, Shannon T Krauss, James P Landers

Abstract read
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In one paragraph

Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed.

  1. Trial
  2. Review
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  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Review
  11. Microfluidic Organ-on-A-chip: A Guide to Biomaterial Choice and Fabrication.International journal of molecular sciences · 2023
    Review
  12. Article
  13. Review
  14. Article
  15. Microfluidic Approaches for Affinity-Based Exosome Separation.International journal of molecular sciences · 2022
    Review
  16. Article
  17. Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Brandon L ThompsonDepartment of Chemistry, University of Virginia, Charlottesville, Virginia, USA.
Yiwen OuyangDepartment of Chemistry, University of Virginia, Charlottesville, Virginia, USA.
Gabriela R M DuarteInstituto de Química, Universidade Federal De Goiás, Goiânia-Go, Brazil.
Emanuel CarrilhoBioanalytical, Microfabrication and Separations Group, Instituto de Química de São Carlos/Universidade de São Paulo, Instituto Nacional de Ciência e Tecnologia de Bioanalítica, São Carlos, Brazil.
Shannon T KraussDepartment of Chemistry, University of Virginia, Charlottesville, Virginia, USA.
James P Landers1] Department of Chemistry, University of Virginia, Charlottesville, Virginia, USA. [2] Department of Mechanical Engineering, University of Virginia, Charlottesville, Virginia, USA. [3] Department of Pathology, University of Virginia, Charlottesville, Virginia, USA.

Funding

Passive Flow Control for Integrated Genomic Analysis MicrochipsR01EB011591 · NIBIB · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI BEGLEY, MATTHEW RICHARD · 2010 to 2013
$1.3M
NIBIB NIH HHS 5R01EB011591-04
6 · The paper itself

Abstract

We describe a technique for fabricating microfluidic devices with complex multilayer architectures using a laser printer, a CO2 laser cutter, an office laminator and common overhead transparencies as a printable substrate via a laser print, cut and laminate (PCL) methodology. The printer toner serves three functions: (i) it defines the microfluidic architecture, which is printed on the overhead transparencies; (ii) it acts as the adhesive agent for the bonding of multiple transparency layers; and (iii) it provides, in its unmodified state, printable, hydrophobic 'valves' for fluidic flow control. By using common graphics software, e.g., CorelDRAW or AutoCAD, the protocol produces microfluidic devices with a design-to-device time of ∼40 min. Devices of any shape can be generated for an array of multistep assays, with colorimetric detection of molecular species ranging from small molecules to proteins. Channels with varying depths can be formed using multiple transparency layers in which a CO2 laser is used to remove the polyester from the channel sections of the internal layers. The simplicity of the protocol, availability of the equipment and substrate and cost-effective nature of the process make microfluidic devices available to those who might benefit most from expedited, microscale chemistry.

Indexed as

Lab-On-A-Chip DevicesPrintingLasers, Gas

Identifiers

PMID25974096

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.