ArticleMolecular oral microbiology2015
Functional profiling in Streptococcus mutans: construction and examination of a genomic collection of gene deletion mutants.
Article in Molecular oral microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 47 citations in OpenAlex.
- Compendium of RNA modifications for bacterial stress adaptation.Microbiology and molecular biology reviews : MMBR · 2026Review
- Monounsaturated fatty acid biosynthesis is critical for streptococcal envelope homeostasis and stress tolerance.Journal of bacteriology · 2026Article
- Development of a spectrophotometric assay for high-throughput screening and mechanistic characterization of glucose-1-phosphate thymidylyltransferase inhibitors.RSC chemical biology · 2026Article
- Epigenomics identifies three sources of DNA methylation inbioRxiv : the preprint server for biology · 2026Article
- Monounsaturated fatty acid biosynthesis is critical for streptococcal envelope homeostasis and stress tolerance.bioRxiv : the preprint server for biology · 2026Article
- A Defined Artificial Saliva Medium Allows Characterization of the Hypothiocyanite Response of Streptococcus sanguinis and Streptococcus mutans.Molecular oral microbiology · 2025Article
- tRNA Modification Landscapes in Streptococci: Shared Losses and Clade-Specific Adaptations.bioRxiv : the preprint server for biology · 2025Article
- Acquired CRISPR spacers and rhamnose-glucose polysaccharide defects confer resistance toMicrobiology (Reading, England) · 2025Article
- Acquired CRISPR spacers and rhamnose-glucose polysaccharide defects confer resistance tobioRxiv : the preprint server for biology · 2025Article
- The antibiofilm effect and mechanism of silver nanowire-modified glass ionomer cement against multi-species oral biofilm.BMC oral health · 2025Article
- In Situ Biofilm Affinity-Based Protein Profiling Identifies the Streptococcal Hydrolase GbpB as the Target of a Carolacton-Inspired Chemical Probe.Journal of the American Chemical Society · 2024Article
- Small RNA SmsR1 modulates acidogenicity and cariogenic virulence by affecting protein acetylation in Streptococcus mutans.PLoS pathogens · 2024Article
- The antimicrobial effect of different vitamin D compounds onJournal of oral microbiology · 2024Article
- Effects of caffeic acid phenethyl ester against multi-species cariogenic biofilms.Folia microbiologica · 2023Article
- Transposon mutagenesis in oralJournal of oral microbiology · 2022Review
- Repression of the TreR transcriptional regulator in Streptococcus mutans by the global regulator, CcpA.FEMS microbiology letters · 2021Article
- Streptococcus mutans SpxA2 relays the signal of cell envelope stress from LiaR to effectors that maintain cell wall and membrane homeostasis.Molecular oral microbiology · 2020Article
- Repurposing the Streptococcus mutans CRISPR-Cas9 System to Understand Essential Gene Function.PLoS pathogens · 2020Article
- Disruption of l-Rhamnose Biosynthesis Results in Severe Growth Defects in Streptococcus mutans.Journal of bacteriology · 2020Article
- Antimicrobial and antibiofilm activities of Casearia sylvestris extracts from distinct Brazilian biomes against Streptococcus mutans and Candida albicans.BMC complementary and alternative medicine · 2019Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
A collection of tagged deletion mutant strains was created in Streptococcus mutans UA159 to facilitate investigation of the aciduric capability of this oral pathogen. Gene-specific barcoded deletions were attempted in 1432 open reading frames (representing 73% of the genome), and resulted in the isolation of 1112 strains (56% coverage) carrying deletions in distinct non-essential genes. As S. mutans virulence is predicated upon the ability of the organism to survive an acidic pH environment, form biofilms on tooth surfaces, and out-compete other oral microflora, we assayed individual mutant strains for the relative fitness of the deletion strain, compared with the parent strain, under acidic and oxidative stress conditions, as well as for their ability to form biofilms in glucose- or sucrose-containing medium. Our studies revealed a total of 51 deletion strains with defects in both aciduricity and biofilm formation. We have also identified 49 strains whose gene deletion confers sensitivity to oxidative damage and deficiencies in biofilm formation. We demonstrate the ability to examine competitive fitness of mutant organisms using the barcode tags incorporated into each deletion strain to examine the representation of a particular strain in a population. Co-cultures of deletion strains were grown either in vitro in a chemostat to steady-state values of pH 7 and pH 5 or in vivo in an animal model for oral infection. Taken together, these data represent a mechanism for assessing the virulence capacity of this pathogenic microorganism and a resource for identifying future targets for drug intervention to promote healthy oral microflora.
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