ArticlePloS one2015
Differential Genes Expression between Fertile and Infertile Spermatozoa Revealed by Transcriptome Analysis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 174 citations in OpenAlex.
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- Proteomic Profiling of Cryopreservation-Induced Alterations in Rhesus Macaque Sperm.Andrology · 2026Article
- Genome-Wide Association Analysis of the Sperm Motility Traits of Jinding Drakes.Animals : an open access journal from MDPI · 2026Article
- The mitochondrial signature of sperm motility loss: a study of mitophagy and apoptosis in asthenozoospermia.Journal of assisted reproduction and genetics · 2026Article
- GDI2 protein: research progress and its mechanisms in diseases.Frontiers in cell and developmental biology · 2026Review
- Transcriptional profiles of the fish parasite Neoechinorhynchus agilis (Acanthocephala) emphasize energetic stress in males and high cell-division activity in females.BMC genomics · 2025Article
- Paternal ribosome biogenesis dysfunction caused by cyclophosphamide-busulfan chemotherapy contributes to early embryonic developmental impairment in mice.Journal of translational medicine · 2025Article
- Review
- Proteomic analysis reveals the difference between the spermatozoa of young and old Sus scrofa.Scientific reports · 2025Article
- Genome-Wide microRNA Expression Profiling in Human Spermatozoa and Its Relation to Sperm Quality.Genes · 2025Article
- RpL38 modulates germ cell differentiation by controlling Bam expression in Drosophila testis.Science China. Life sciences · 2024Article
- A narrative review of sperm selection technology for assisted reproduction techniques.Translational andrology and urology · 2024Review
- Genetic Diversity, Selection Signatures, and Genome-Wide Association Study Identify Candidate Genes Related to Litter Size in Hu Sheep.International journal of molecular sciences · 2024Article
- The Beak of Eukaryotic Ribosomes: Life, Work and Miracles.Biomolecules · 2024Review
- Sperm epigenetics and male infertility: unraveling the molecular puzzle.Human genomics · 2024Review
- Analysis of 206 whole-genome resequencing reveals selection signatures associated with breed-specific traits in Hu sheep.Evolutionary applications · 2024Article
- Genome-wide association study identifies genomic regions associated with key reproductive traits in Korean Hanwoo cows.BMC genomics · 2024Article
- Semen proteome and transcriptome of the endangered black-footed ferret (Mustela nigripes) show association with the environment and fertility outcome.Scientific reports · 2024Article
- Transcriptome- and proteome-wide effects of a circular RNA encompassing four early exons of the spinal muscular atrophy genes.Research square · 2024Article
- Immunophenotype profile by flow cytometry reveals different subtypes of extracellular vesicles in porcine seminal plasma.Cell communication and signaling : CCS · 2024Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIt was believed earlier that spermatozoa have no traces of RNA because of loss of most of the cytoplasm. Recent studies have revealed the presence of about 3000 different kinds of mRNAs in ejaculated spermatozoa. However, the correlation of transcriptome profile with infertility remains obscure.
methodsTotal RNA from sperm (after exclusion of somatic cells) of 60 men consisting of individuals with known fertility (n=20), idiopathic infertility (normozoospermic patients, n=20), and asthenozoospermia (n=20) was isolated. After RNA quality check on Bioanalyzer, AffymetrixGeneChip Human Gene 1.0 ST Array was used for expression profiling, which consisted of >30,000 coding transcripts and >11,000 long intergenic non-coding transcripts.
resultsComparison between all three groups revealed that two thousand and eighty one transcripts were differentially expressed. Analysis of these transcripts showed that some transcripts [ribosomal proteins (RPS25, RPS11, RPS13, RPL30, RPL34, RPL27, RPS5), HINT1, HSP90AB1, SRSF9, EIF4G2, ILF2] were up-regulated in the normozoospermic group, but down-regulated in the asthenozoospermic group in comparison to the control group. Some transcripts were specific to the normozoospermic group (up-regulated: CAPNS1, FAM153C, ARF1, CFL1, RPL19, USP22; down-regulated: ZNF90, SMNDC1, c14orf126, HNRNPK), while some were specific to the asthenozoospermic group (up-regulated: RPL24, HNRNPM, RPL4, PRPF8, HTN3, RPL11, RPL28, RPS16, SLC25A3, C2orf24, RHOA, GDI2, NONO, PARK7; down-regulated: HNRNPC, SMARCAD1, RPS24, RPS24, RPS27A, KIFAP3). A number of differentially expressed transcripts in spermatozoa were related to reproduction (n = 58) and development (n= 210). Some of these transcripts were related to heat shock proteins (DNAJB4, DNAJB14), testis specific genes (TCP11, TESK1, TSPYL1, ADAD1), and Y-chromosome genes (DAZ1, TSPYL1).
conclusionA complex RNA population in spermatozoa consisted of coding and non-coding RNAs. A number of transcripts that participate in a host of cellular processes, including reproduction and development were differentially expressed between fertile and infertile individuals. Differences between comparison groups suggest that sperm RNA has strong potential of acting as markers for fertility evaluation.
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