ArticlePlant biotechnology journal2016
Identification and functional characterization of soybean root hair microRNAs expressed in response to Bradyrhizobium japonicum infection.
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Small but big player: the important role of microRNAs in legume crops.Molecular genetics and genomics : MGG · 2026Review
- Epigenetics in the modern era of crop improvements.Science China. Life sciences · 2025Review
- Non-conserved microRNAs and their roles in plants: the case for legumes.Plant biology (Stuttgart, Germany) · 2025Review
- A Comprehensive Transcriptome Atlas Reveals the Crucial Role of LncRNAs in Maintaining Nodulation Homeostasis in Soybean.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Article
- Clone and Function Verification of the OPR gene in Brassica napus Related to Linoleic Acid Synthesis.BMC plant biology · 2022Article
- Progress in soybean functional genomics over the past decade.Plant biotechnology journal · 2022Review
- A Multi-Level Iterative Bi-Clustering Method for Discovering miRNA Co-regulation Network of Abiotic Stress Tolerance in Soybeans.Frontiers in plant science · 2022Article
- Using the Knowledge of Post-transcriptional Regulations to Guide Gene Selections for Molecular Breeding in Soybean.Frontiers in plant science · 2022Review
- Understanding plant-microbe interaction of rice and soybean with two contrasting diazotrophic bacteria through comparative transcriptome analysis.Frontiers in plant science · 2022Article
- Review
- Title: Hypermethylation of miRNA Genes During Nodule Development.Frontiers in molecular biosciences · 2021Article
- miRNAomic Approach to Plant Nitrogen Starvation.International journal of genomics · 2021Review
- High-Throughput Sequencing and Expression Analysis Suggest the Involvement ofInternational journal of molecular sciences · 2020Article
- Development of a fast and efficient root transgenic system for functional genomics and genetic engineering in peach.Scientific reports · 2020Article
- Gene Expression in Nitrogen-Fixing Symbiotic Nodule Cells inThe Plant cell · 2020Review
- Legumes display common and host-specific responses to the rhizobial cellulase CelC2 during primary symbiotic infection.Scientific reports · 2019Article
- Integrated microRNA and transcriptome profiling reveals a miRNA-mediated regulatory network of embryo abortion under calcium deficiency in peanut (Arachis hypogaea L.).BMC genomics · 2019Article
- Homeostasis in the soybean miRNA396-GRF network is essential for productive soybean cyst nematode infections.Journal of experimental botany · 2019Article
- Transcriptional Reprogramming of Legume Genomes: Perspective and Challenges Associated With Single-Cell and Single Cell-Type Approaches During Nodule Development.Frontiers in plant science · 2018Article
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Authors and funding
16 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three soybean [Glycine max (L) Merr.] small RNA libraries were generated and sequenced using the Illumina platform to examine the role of miRNAs during soybean nodulation. The small RNA libraries were derived from root hairs inoculated with Bradyrhizobium japonicum (In_RH) or mock-inoculated with water (Un_RH), as well as from the comparable inoculated stripped root samples (i.e. inoculated roots with the root hairs removed). Sequencing of these libraries identified a total of 114 miRNAs, including 22 novel miRNAs. A comparison of miRNA abundance among the 114 miRNAs identified 66 miRNAs that were differentially expressed between root hairs and stripped roots, and 48 miRNAs that were differentially regulated in infected root hairs in response to B. japonicum when compared to uninfected root hairs (P ≤ 0.05). A parallel analysis of RNA ends (PARE) library was constructed and sequenced to reveal a total of 405 soybean miRNA targets, with most predicted to encode transcription factors or proteins involved in protein modification, protein degradation and hormone pathways. The roles of gma-miR4416 and gma-miR2606b during nodulation were further analysed. Ectopic expression of these two miRNAs in soybean roots resulted in significant changes in nodule numbers. miRNA target information suggested that gma-miR2606b regulates a Mannosyl-oligosaccharide 1, 2-alpha-mannosidase gene, while gma-miR4416 regulates the expression of a rhizobium-induced peroxidase 1 (RIP1)-like peroxidase gene, GmRIP1, during nodulation.
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