Evidence map›Paper›PMID 25973637›Full record

ArticleAnalytical chemistry2015

Polymer Coatings in 3D-Printed Fluidic Device Channels for Improved Cellular Adherence Prior to Electrical Lysis.

Bethany C Gross, Kari B Anderson, Jayda E Meisel, Megan I McNitt, Dana M Spence

Open access · greenAbstract read
In one paragraph

Article in Analytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
4.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 57 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. Review
  7. Review
  8. Review
  9. Article
  10. 3D-printed Bioreactors forInternational journal of bioprinting · 2020
    Review
  11. Review
  12. The Evolution of Polystyrene as a Cell Culture Material.Tissue engineering. Part B, Reviews · 2018
    Review
  13. Article
  14. Article
  15. Review
  16. 3D-printed Microfluidic Devices: Fabrication, Advantages and Limitations-a Mini Review.Analytical methods : advancing methods and applications · 2016
    Article
  17. 3D-printed bioanalytical devices.Nanotechnology · 2016
    Review
  18. Article
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Bethany C GrossDepartment of Chemistry, Michigan State University, East Lansing, Michigan 48823, United States.
Kari B AndersonDepartment of Chemistry, Michigan State University, East Lansing, Michigan 48823, United States.
Jayda E MeiselDepartment of Chemistry, Michigan State University, East Lansing, Michigan 48823, United States.
Megan I McNittDepartment of Chemistry, Michigan State University, East Lansing, Michigan 48823, United States.
Dana M SpenceDepartment of Chemistry, Michigan State University, East Lansing, Michigan 48823, United States.
Michigan State University · US

Funding

Spatial SILAC: Examining the Proteome in 3D Cell CulturesR01GM110406 · NIGMS · UNIVERSITY OF NOTRE DAME · PI HUMMON, AMANDA B. · 2014 to 2022
$3.0M
Modular Microfluidics as an Enhanced Bioanalytical ToolR21EB016379 · NIBIB · MICHIGAN STATE UNIVERSITY · PI SPENCE, DANA M · 2013 to 2014
$393k
NIBIB NIH HHS 1R21EB016379NIBIB NIH HHS R21 EB016379NIGMS NIH HHS 1R01GM110406NIGMS NIH HHS R01 GM110406
6 · The paper itself

Abstract

This paper describes the design and fabrication of a polyjet-based three-dimensional (3D)-printed fluidic device where poly(dimethylsiloxane) (PDMS) or polystyrene (PS) were used to coat the sides of a fluidic channel within the device to promote adhesion of an immobilized cell layer. The device was designed using computer-aided design software and converted into an .STL file prior to printing. The rigid, transparent material used in the printing process provides an optically transparent path to visualize endothelial cell adherence and supports integration of removable electrodes for electrical cell lysis in a specified portion of the channel (1 mm width × 0.8 mm height × 2 mm length). Through manipulation of channel geometry, a low-voltage power source (500 V max) was used to selectively lyse adhered endothelial cells in a tapered region of the channel. Cell viability was maintained on the device over a 5 day period (98% viable), though cell coverage decreased after day 4 with static media delivery. Optimal lysis potentials were obtained for the two fabricated device geometries, and selective cell clearance was achieved with cell lysis efficiencies of 94 and 96%. The bottleneck of unknown surface properties from proprietary resin use in fabricating 3D-printed materials is overcome through techniques to incorporate PDMS and PS.

Indexed as

Microfluidic Analytical TechniquesPrinting, Three-DimensionalCell AdhesionCell ExtractsCell SurvivalCoated Materials, BiocompatibleDimethylpolysiloxanesElectrodesEndothelial CellsEquipment DesignHumansPolystyrenesbaysilonCell ExtractsCoated Materials, BiocompatibleDimethylpolysiloxanesPolystyrenes

Identifiers

PMID25973637
PMCPMC5362108
OpenAlexW2475325728

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.