Evidence map›Paper›PMID 25973041›Full record

ArticleInternational journal of clinical and experimental pathology2015

Downregulated miR-29c correlates with increased BACE1 expression in sporadic Alzheimer's disease.

Xiaofeng Lei, Lijian Lei, Zhelin Zhang, Zhiqing Zhang, Yan Cheng

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers, 6 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
72citing papers in PubMed, 6 pooled it
3.7field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

72 citing papers in PubMed, 6 syntheses or guidelines pooled it, 115 citations in OpenAlex.

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  16. An Update on Neuroaging on Earth and in Spaceflight.International journal of molecular sciences · 2025
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12 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Xiaofeng LeiDepartment of Neurology Medicine, Tianjin Medical University General Hospital Tanjin 300052, China ; Department of Neurology Medicine, Tianjin 4th Center Hospital Tianjin 300140, China.
Lijian LeiSchool of Public Health, Shanxi Medical University Taiyuan, Shanxi 030001, China.
Zhelin ZhangDepartment of Neurology Medicine, Tianjin Medical University General Hospital Tanjin 300052, China.
Zhiqing ZhangDepartment of Neurology Medicine, Tianjin 4th Center Hospital Tianjin 300140, China.
Yan ChengDepartment of Neurology Medicine, Tianjin Medical University General Hospital Tanjin 300052, China.
Tianjin Medical University General Hospital · CNShanxi Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBeta-site Amyloid precursor protein Cleaving Enzyme 1 (BACE1) is conceived as a potential target for therapies against Alzheimer disease (AD) which is characterized by the accumulation of plaques formed of short β-amyloid (APPβ) peptides. Recently, such microRNAs, as miR-29a, miR-29b-1 have been shown to correlate with abnormally high levels of BACE1 and APPβ in sporadic AD.

methodsIn order to confirm whether miR-29c correlates with the BACE1 upregulation in sporadic AD, we firstly evaluated the expression of miR-29c and BACE1, the APPβ accumulation in sporadic AD brain tissues and analyzed the correlation of miR-29c with BACE1. Then we determined the regulation of miR-29c in human heuroblastoma SH-SY5Y cells on the BACE1 expression and APPβ accumulation. And finally we determined the targeting to 3' UTR of BACE1 by miR-29c by a luciferase reporter.

resultsIt was demonstrated that miR-29c was downregulated in sporadic AD brains, in an association with an upregulation of BACE1 in both mRNA and protein level of BACE1, and also an elevated APPβ accumulation. And the manipulated high level of miR-29c with miR-29c mimics transfection significantly reduced the protein level of BACE1 and APPβ accumulation in human neuroblastoma SH-SY5Y cells. Further luciferase reporter assay demonstrated that miR-29c targets the 3' UTR of BACE1 and downregulated the BACE1 in HEK293 cells.

conclusionPresent study indicated that miR-29c was downregulated in sporadic AD brains, and it targeted the 3' UTR of BACE1, reduced the BACE1 expression, and downregulated the APPβ accumulation in vitro.

Indexed as

AgedAged, 80 and overAlzheimer DiseaseAmyloid beta-PeptidesAmyloid Precursor Protein SecretasesAspartic Acid EndopeptidasesBlotting, WesternDown-RegulationFemaleGene Expression RegulationHumansMaleMicroRNAsReal-Time Polymerase Chain ReactionTransfectionUp-RegulationAmyloid beta-PeptidesAmyloid Precursor Protein SecretasesAspartic Acid EndopeptidasesBACE1 protein, humanMicroRNAsMIRN29a microRNA, humanAlzheimer’s diseaseAPPβ accumulationBACE1miR-29c

Identifiers

PMID25973041
PMCPMC4396232
OpenAlexW307728228

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.