ArticleBiology of reproduction2015
Rhox8 Ablation in the Sertoli Cells Using a Tissue-Specific RNAi Approach Results in Impaired Male Fertility in Mice.
Article in Biology of reproduction, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Acute stress induction downregulates RHOX-5 gene expression causing altered fertility indices in MALE Wistar rats.JBRA assisted reproduction · 2026Article
- Conditional ablation of Insr and Igf1r using Esr2-Cre leads to abnormal ovarian follicle development and infertility in mice†.Biology of reproduction · 2025Article
- RHOX Homeobox Transcription Factor Regulation ofCells · 2025Article
- Rhox8 homeobox gene ablation leads to rete testis abnormality and male subfertility in mice†.Biology of reproduction · 2023Article
- Mammal Reproductive Homeobox (Rhox) Genes: An Update of Their Involvement in Reproduction and Development.Genes · 2023Review
- Review
- Interaction between mono-(2-ethylhexyl) phthalate and retinoic acid alters Sertoli cell development during fetal mouse testis cord morphogenesis.Current research in toxicology · 2022Article
- Concordant Androgen-Regulated Expression of Divergent Rhox5 Promoters in Sertoli Cells.Endocrinology · 2022Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Insulin signaling is an essential regulator of endometrial proliferation and implantation in mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- hnRNPU in Sertoli cells cooperates with WT1 and is essential for testicular development by modulating transcriptional factorsTheranostics · 2021Article
- WTAP Function in Sertoli Cells Is Essential for Sustaining the Spermatogonial Stem Cell Niche.Stem cell reports · 2020Article
- Periovulatory insulin signaling is essential for ovulation, granulosa cell differentiation, and female fertility.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Toward Development of the Male Pill: A Decade of Potential Non-hormonal Contraceptive Targets.Frontiers in cell and developmental biology · 2020Review
- Bisamide Derivative of Dicarboxylic Acid Contributes to Restoration of Testicular Tissue Function and Influences Spermatogonial Stem Cells in Metabolic Disorders.Frontiers in cell and developmental biology · 2020Article
- The human RHOX gene cluster: target genes and functional analysis of gene variants in infertile men.Human molecular genetics · 2016Article
- Rhox13 is required for a quantitatively normal first wave of spermatogenesis in mice.Reproduction (Cambridge, England) · 2016Article
- The Homeobox Transcription Factor RHOX10 Drives Mouse Spermatogonial Stem Cell Establishment.Cell reports · 2016Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The reproductive homeobox X-linked, Rhox, genes encode transcription factors that are selectively expressed in reproductive tissues. While there are 33 Rhox genes in mice, only Rhox and Rhox8 are expressed in Sertoli cells, suggesting that they may regulate the expression of somatic-cell gene products crucial for germ cell development. We previously characterized Rhox5-null mice, which are subfertile, exhibiting excessive germ cell apoptosis and compromised sperm motility. To assess the role of Rhox8 in Sertoli cells, we used a tissue-specific RNAi approach to knockdown RHOX8 in vivo, in which the Rhox5 promoter was used to drive Rhox8-siRNA transgene expression in the postnatal Sertoli cells. Western and immunohistochemical analysis confirmed Sertoli-specific knockdown of RHOX8. However, other Sertoli markers, Gata1 and Rhox5, maintained normal expression patterns, suggesting that the knockdown was specific. Interestingly, male RHOX8-knockdown animals showed significantly reduced spermatogenic output, increased germ cell apoptosis, and compromised sperm motility, leading to impaired fertility. Importantly, our results revealed that while some RHOX5-dependent factors were also misregulated in Sertoli cells of RHOX8-knockdown animals, the majority were not, and novel putative RHOX8-regulated genes were identified. This suggests that while reduction in levels of RHOX5 and RHOX8 in Sertoli cells elicits similar phenotypes, these genes are not entirely redundant. Taken together, our study underscores the importance of Rhox genes in male fertility and suggests that Sertoli cell-specific expression of Rhox5 and Rhox8 is critical for complete male fertility.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.