Evidence map›Paper›PMID 25971815›Full record

Trial reportLipids in health and disease2015

Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: an open-label intervention study.

Simone Schmidt, Janina Willers, Sabine Riecker, Katharina Möller, Jan Philipp Schuchardt, Andreas Hahn

Open access · goldAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Lipids in health and disease, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Simone SchmidtInstitute of Food Science and Human Nutrition, Leibniz University of Hannover, Am Kleinen Felde 30, 30167, Hannover, Germany. schmidt.simone@arcor.de.
Janina WillersInstitute of Food Science and Human Nutrition, Leibniz University of Hannover, Am Kleinen Felde 30, 30167, Hannover, Germany. willers@nutrition.uni-hannover.de.
Sabine RieckerInstitute of Food Science and Human Nutrition, Leibniz University of Hannover, Am Kleinen Felde 30, 30167, Hannover, Germany. riecker@nutrition.uni-hannover.de.
Katharina MöllerInstitute of Food Science and Human Nutrition, Leibniz University of Hannover, Am Kleinen Felde 30, 30167, Hannover, Germany. moeller@nutrition.uni-hannover.de.
Jan Philipp SchuchardtInstitute of Food Science and Human Nutrition, Leibniz University of Hannover, Am Kleinen Felde 30, 30167, Hannover, Germany. schuchardt@nutrition.uni-hannover.de.
Andreas HahnInstitute of Food Science and Human Nutrition, Leibniz University of Hannover, Am Kleinen Felde 30, 30167, Hannover, Germany. hahn@nutrition.uni-hannover.de.
Leibniz University Hannover · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundOmega-3 polyunsaturated fatty acids (n-3 PUFAs) show beneficial effects on cardiovascular health and cognitive functions, but the underlying molecular mechanisms are not completely understood. Because of the fact that cytoskeleton dynamics affect almost every cellular process, the regulation of cytoskeletal dynamics could be a new pathway by which n-3 PUFAs exert their effects on cellular level.

methodsA 12-week open-label intervention study with 12 healthy men was conducted to determine the effects of 2.7 g/d n-3 PUFA on changes in mRNA expression of cytoskeleton-associated genes by quantitative real-time PCR in whole blood. Furthermore, the actin content in red blood cells was analyzed by immunofluorescence imaging.

resultsN-3 PUFA supplementation resulted in a significant down-regulation of cytoskeleton-associated genes, in particular three GTPases (RAC1, RHOA, CDC42), three kinases (ROCK1, PAK2, LIMK), two Wiskott-Aldrich syndrome proteins (WASL, WASF2) as well as actin related protein 2/3 complex (ARPC2, ARPC3) and cofilin (CFL1). Variability in F-actin content between subjects was high; reduced actin content was only reduced within group evaluation.

conclusionsReduced cytoskeleton-associated gene expression after n-3 PUFA supplementation suggests that regulation of cytoskeleton dynamics might be an additional way by which n-3 PUFAs exert their cellular effects. Concerning F-actin, this analysis did not reveal unmistakable results impeding a generalized conclusion.

Indexed as

Actin-Related Protein 2-3 ComplexAdultcdc42 GTP-Binding ProteinCofilin 1CytoskeletonDown-RegulationFatty Acids, Omega-3Fluorescent Antibody TechniqueGene ExpressionHumansLim KinasesMalep21-Activated Kinasesrac1 GTP-Binding ProteinReal-Time Polymerase Chain ReactionrhoA GTP-Binding ProteinActin-Related Protein 2-3 ComplexARPC2 protein, humanARPC3 protein, humancdc42 GTP-Binding ProteinCFL1 protein, humanCofilin 1Fatty Acids, Omega-3LIMK1 protein, humanLim Kinasesp21-Activated KinasesPAK2 protein, humanrac1 GTP-Binding ProteinRAC1 protein, humanrhoA GTP-Binding ProteinRHOA protein, humanrho-Associated KinasesROCK1 protein, humanWASF2 protein, humanWASL protein, humanWiskott-Aldrich Syndrome Protein FamilyWiskott-Aldrich Syndrome Protein, Neuronal

Identifiers

PMID25971815
PMCPMC4429932
OpenAlexW2168197975

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.