ArticleMolecular biology of the cell2015
A novel tropomyosin isoform functions at the mitotic spindle and Golgi in Drosophila.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Functional redundancy and formin-isoform independent localization of tropomyosin paralogs in Saccharomyces cerevisiae.PLoS genetics · 2025Article
- Actin network evolution as a key driver of eukaryotic diversification.Journal of cell science · 2024Review
- Alternative splicing of a single exon causes a major impact on the affinity ofFrontiers in cell and developmental biology · 2023Article
- Targeting the cytoskeleton against metastatic dissemination.Cancer metastasis reviews · 2021Review
- Article
- Actin-tropomyosin distribution in non-muscle cells.Journal of muscle research and cell motility · 2020Review
- Tropomyosin isoforms differentially affect muscle contractility in the head and body regions of the nematode Caenorhabditis elegans.Molecular biology of the cell · 2018Article
- Parallel assembly of actin and tropomyosin, but not myosin II, duringJournal of cell science · 2018Article
- Networks that link cytoskeletal regulators and diaphragm proteins underpin filtration function in Drosophila nephrocytes.Experimental cell research · 2018Article
- On-target action of anti-tropomyosin drugs regulates glucose metabolism.Scientific reports · 2018Article
- Article
- A new isoform of Drosophila non-muscle Tropomyosin 1 interacts with Kinesin-1 and functions in oskar mRNA localization.Journal of cell science · 2016Article
- Analysis of biophysical and functional consequences of tropomyosin-fluorescent protein fusions.FEBS letters · 2016Article
- An Atypical Tropomyosin in Drosophila with Intermediate Filament-like Properties.Cell reports · 2016Article
- A small molecule inhibitor of tropomyosin dissociates actin binding from tropomyosin-directed regulation of actin dynamics.Scientific reports · 2016Article
- Comparative analysis of tools for live cell imaging of actin network architecture.Bioarchitecture · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Most eukaryotic cells express multiple isoforms of the actin-binding protein tropomyosin that help construct a variety of cytoskeletal networks. Only one nonmuscle tropomyosin (Tm1A) has previously been described in Drosophila, but developmental defects caused by insertion of P-elements near tropomyosin genes imply the existence of additional, nonmuscle isoforms. Using biochemical and molecular genetic approaches, we identified three tropomyosins expressed in Drosophila S2 cells: Tm1A, Tm1J, and Tm2A. The Tm1A isoform localizes to the cell cortex, lamellar actin networks, and the cleavage furrow of dividing cells--always together with myosin-II. Isoforms Tm1J and Tm2A colocalize around the Golgi apparatus with the formin-family protein Diaphanous, and loss of either isoform perturbs cell cycle progression. During mitosis, Tm1J localizes to the mitotic spindle, where it promotes chromosome segregation. Using chimeras, we identified the determinants of tropomyosin localization near the C-terminus. This work 1) identifies and characterizes previously unknown nonmuscle tropomyosins in Drosophila, 2) reveals a function for tropomyosin in the mitotic spindle, and 3) uncovers sequence elements that specify isoform-specific localizations and functions of tropomyosin.
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