ArticleGenetics2015
Chromosome-Specific Painting in Cucumis Species Using Bulked Oligonucleotides.
Article in Genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 123 papers.
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Who cites it
123 citing papers in PubMed, 229 citations in OpenAlex.
- An integrated molecular cytogenetic platform enables precise characterization of Thinopyrum ponticum chromatin and its structural modifications in wheat background.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Chromosome painting in plants: history and future perspectives.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026Review
- Chromosome-scale genomes and population resequencing resolve subgenome diversity and halophyte adaptation in Salicornia.Nature communications · 2026Article
- Research Progress in the Cytogenetics of Sweetpotato and Its Wild Relatives.Plants (Basel, Switzerland) · 2026Review
- Review
- Comparative Oligo-FISH Mapping Illuminates Chromosomal Evolution Among Rutaceae Species Diverged Over 50 Million Years.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Stable resynthesized Brassica napus lines show similar meiotic behaviour to established B. napus.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026Article
- Origin of small chromosome A08 and genome evolution of Arachis species.Nature communications · 2026Article
- Cytogenomic and phylogenomic evidence for new infrageneric relationships in Macroptilium (Benth.) beans.Annals of botany · 2026Article
- Human selection maintains karyotype integrity of highly unstable genomic cultivated autotetraploid potato (Science advances · 2025Article
- Near-complete genome assembly of allotetraploid Erianthus rockii reveals unique chromosome evolution and lineage-divergence trajectories in the Saccharum complex.Plant communications · 2025Article
- Whole Genome Development of Specific Alien-Chromosome Oligo (SAO) Markers for Wild Peanut Chromosomes Based on Chorus2.Plants (Basel, Switzerland) · 2025Article
- Chromosome-level genome assembly and Oligo-FISH confirmation of chromosome models for the Tasmanian snow skink Carinascincus ocellatus (Scincidae, Eugongylinae).BMC biology · 2025Article
- Identification of a Novel Rice Chromosomal Translocation Line that Could Cause the Heterozygote Semi-Sterility and be Overcome by Genomic Duplication.Rice (New York, N.Y.) · 2025Article
- Thriving or Withering? Plant Molecular Cytogenetics in the First Quarter of the 21st Century.International journal of molecular sciences · 2025Review
- "End-to-End Chromosome Fusion" as the Main Driver of Descending Dysploidy inPlants (Basel, Switzerland) · 2025Article
- Epigenetic state and gene expression remain stable after CRISPR/Cas-mediated chromosomal inversions.The New phytologist · 2025Article
- Development of a set of monosomic alien addition lines from Gossypium raimondii in Gossypium hirsutum toward breeding applications in cotton.BMC plant biology · 2025Article
- Cross-species chromosome painting offers new insights into the phylogenetic relationships among 16 representative species of Ipomoeeae.Frontiers in plant science · 2025Article
- A cost-effective oligo-based barcode system for chromosome identification in longan and lychee.Horticulture research · 2025Article
63 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosome-specific painting is a powerful technique in molecular cytogenetic and genome research. We developed an oligonucleotide (oligo)-based chromosome painting technique in cucumber (Cucumis sativus) that will be applicable in any plant species with a sequenced genome. Oligos specific to a single chromosome of cucumber were identified using a newly developed bioinformatic pipeline and then massively synthesized de novo in parallel. The synthesized oligos were amplified and labeled with biotin or digoxigenin for use in fluorescence in situ hybridization (FISH). We developed three different probes with each containing 23,000-27,000 oligos. These probes spanned 8.3-17 Mb of DNA on targeted cucumber chromosomes and had the densities of 1.5-3.2 oligos per kilobases. These probes produced FISH signals on a single cucumber chromosome and were used to paint homeologous chromosomes in other Cucumis species diverged from cucumber for up to 12 million years. The bulked oligo probes allowed us to track a single chromosome in early stages during meiosis. We were able to precisely map the pairing between cucumber chromosome 7 and chromosome 1 of Cucumis hystrix in a F1 hybrid. These two homeologous chromosomes paired in 71% of prophase I cells but only 25% of metaphase I cells, which may provide an explanation of the higher recombination rates compared to the chiasma frequencies between homeologous chromosomes reported in plant hybrids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.