ArticlePlant physiology2015
Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic Protein Interaction Studies.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed.
- COJournal of integrative plant biology · 2026Article
- HDA15-Mediated Deacetylation of GPX1 Inhibits Its Nuclear Translocation and Increases Osmotic Stress Sensitivity in Rice.International journal of molecular sciences · 2026Article
- Mapping the dynamic plant interactome: from in vitro assays to in vivo quantitative approaches.Plant methods · 2026Review
- Protein-protein interaction detection in plants: from classical approaches to future perspectives.Biologia futura · 2026Review
- A guard cell carbonic anhydrase binds and regulates SLAC1 separate from its catalytic activity.Nature communications · 2026Article
- Domain architecture of plant eukaryotic translation initiation factor 3 subunit E governs interaction with translational cis-elements to regulatepollen tube growth.The Plant cell · 2026Article
- A phosphoinositide-mediated switch of GET pathway receptor dimerization inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- ERAD machinery controls the conditional turnover of PIN-LIKES in plants.Science advances · 2025Article
- TkSRPP3/4 interactors TkGGR1 and TkLIL3 link plastid-like organelles with isoprenoid metabolism in Taraxacum koksaghyz latex.Plant cell reports · 2025Article
- Pupylation-based proximity labeling reveals regulatory factors in cellulose biosynthesis in Arabidopsis.Nature communications · 2025Article
- FRET-FLIM for the Study of Protein-Protein Interactions Underpinning Mitosis Checkpoints.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Interaction Dynamics of Plant-Specific Insert Domains fromMolecules (Basel, Switzerland) · 2024Article
- MAC3A and MAC3B mediate degradation of the transcription factor ERF13 and thus promote lateral root emergence.The Plant cell · 2024Article
- The TaSnRK1-TabHLH489 module integrates brassinosteroid and sugar signalling to regulate the grain length in bread wheat.Plant biotechnology journal · 2024Article
- NKS1/ELMO4 is an integral protein of a pectin synthesis protein complex and maintains Golgi morphology and cell adhesion inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Group VII ethylene response factors forming distinct regulatory loops mediate submergence responses.Plant physiology · 2024Article
- Gene Stacking and Stoichiometric Expression of ER-Targeted Constructs Using "2A" Self-Cleaving Peptides.Methods in molecular biology (Clifton, N.J.) · 2024Article
- 2 in 1 Vectors Improve in Planta BiFC and FRET Analysis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Recombinant expression and subcellular targeting of the particulate methane monooxygenase (pMMO) protein components in plants.Scientific reports · 2023Article
- Analyzing Protein-Protein Interactions Using the Split-Ubiquitin System.Methods in molecular biology (Clifton, N.J.) · 2023Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
Abstract
Fluorescence-based protein-protein interaction techniques are vital tools for understanding in vivo cellular functions on a mechanistic level. However, only under the condition of highly efficient (co)transformation and accumulation can techniques such as Förster resonance energy transfer (FRET) realize their potential for providing highly accurate and quantitative interaction data. FRET as a fluorescence-based method unifies several advantages, such as measuring in an in vivo environment, real-time context, and the ability to include transient interactions as well as detecting the mere proximity of proteins. Here, we introduce a novel vector set that incorporates the benefit of the recombination-based 2in1 cloning system with the latest state-of-the-art fluorescent proteins for optimal coaccumulation and FRET output studies. We demonstrate its utility across a range of methods. Merging the 2in1 cloning system with new-generation FRET fluorophore pairs allows for enhanced detection, speeds up the preparation of clones, and enables colocalization studies and the identification of meaningful protein-protein interactions in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.