ArticleMolecular and cellular biochemistry2015
Sfrp2 is a transcriptional target of SREBP-1 in mouse chondrogenic cells.
Article in Molecular and cellular biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 3 citations in OpenAlex.
- ER-resident proteins are key players in cartilage and bone homeostasis.Frontiers in cell and developmental biology · 2025Review
- Increased Secreted Frizzled-Related Protein 2 in Hypertension-Induced Left Ventricular Remodeling.Reviews in cardiovascular medicine · 2024Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Secreted frizzled-related protein 2 (Sfrp2) is highly expressed in developing limbs and is associated with skeletal malformation such as Brachydactyly and Syndactyly. However, the mechanism by which Sfrp2 gene was transcriptionally regulated in chondrogenic cells is largely unknown. Here, we found that sterol regulatory element binding protein-1 (SREBP-1) regulated transcriptional activation of the Sfrp2 gene in chondrogenic cells. Overexpression of SREBP-1 led to stimulation of Sfrp2 promoter activity and increase of Sfrp2 mRNA in chondrogenic ATDC5 cells. Reporter gene assays using deleted Sfrp2 promoter constructs showed that the position between -1150 and -840 is required for SREBP-1-mediated Sfrp2 promoter activity. Mutagenesis analysis showed that the SREBP-1-binding site at nucleotide -935 to -926 is a functional motif for Sfrp2 transcription. Promoter enzyme immunoassay and chromatin immunoprecipitation assay demonstrated that SREBP-1 binds directly to the SREBP-1-binding motif in the mouse Sfrp2 promoter. Thus, these results demonstrated that SREBP-1 acts as a positive regulator of Sfrp2 transcription in chondrogenic cells.
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