ArticleThe Biochemical journal1989
Analysis of the water-soluble products of phosphatidylcholine breakdown by ion-exchange chromatography. Bombesin and TPA (12-O-tetradecanoylphorbol 13-acetate) stimulate choline generation in Swiss 3T3 cells by a common mechanism.
Article in The Biochemical journal, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 189 citations in OpenAlex.
- Loss of Ypk1, the yeast homolog to the human serum- and glucocorticoid-induced protein kinase, accelerates phospholipase B1-mediated phosphatidylcholine deacylation.The Journal of biological chemistry · 2014Article
- Glycerophosphocholine utilization by Candida albicans: role of the Git3 transporter in virulence.The Journal of biological chemistry · 2013Article
- Human prostate cell lines from normal and tumourigenic epithelia differ in the pattern and control of choline lipid headgroups released into the medium on stimulation of protein kinase C.British journal of cancer · 2011Article
- Cell wall integrity MAPK pathway is essential for lipid homeostasis.The Journal of biological chemistry · 2008Article
- Effects of flutamide on [methyl-(3)h]-choline uptake in human prostate cancer-3 cells: a pilot study.Current therapeutic research, clinical and experimental · 2007Article
- Inhibition of Plasmodium falciparum choline kinase by hexadecyltrimethylammonium bromide: a possible antimalarial mechanism.Antimicrobial agents and chemotherapy · 2007Article
- Regulation of activity in vitro and in vivo of three phospholipases B from Saccharomyces cerevisiae.The Biochemical journal · 2005Article
- Phosphatidylcholine metabolism in nuclei of phorbol ester-activated LA-N-1 neuroblastoma cells.Neurochemical research · 2000Article
- Ionotrophic 5-hydroxytryptamine type 3 receptor activates the protein kinase C-dependent phospholipase D pathway in human T-cells.The Biochemical journal · 1999Article
- Characterization of the regulation of phospholipase D activity in the detergent-insoluble fraction of HL60 cells by protein kinase C and small G-proteins.The Biochemical journal · 1999Article
- Extracellular sphingosine 1-phosphate stimulates formation of ethanolamine from phosphatidylethanolamine: modulation of sphingosine 1-phosphate-induced mitogenesis by ethanolamine.The Biochemical journal · 1997Article
- A pathway in the yeast cell division cycle linking protein kinase C (Pkc1) to activation of Cdc28 at START.The EMBO journal · 1996Article
- Diversity of roles of protein kinase C alpha in the proliferation of Swiss 3T3 cells.The Biochemical journal · 1996Article
- Potentiated bradykinin-induced increase of 1,2-diacylglycerol generation and phospholipase D activity in human senescent fibroblasts.The Biochemical journal · 1995Article
- Phorbol ester selectively stimulates the phospholipase D-mediated hydrolysis of phosphatidylethanolamine in multidrug-resistant MCF-7 human breast carcinoma cells.The Biochemical journal · 1994Article
- Selective down-regulation of protein kinase c-epsilon by carcinogens does not prevent stimulation of phospholipase D by phorbol ester and platelet-derived growth factor.The Biochemical journal · 1994Article
- Regulation by hypoxia of endothelin-1-stimulated phospholipase D activity in sheep pulmonary artery cultured smooth muscle cells.British journal of pharmacology · 1994Article
- Analysis of hydrolytic products from choline-labeled host cell phospholipids during growth of Rickettsia prowazekii.Infection and immunity · 1994Article
- Rapid desensitization and resensitization of bombesin-stimulated phospholipase D activity in Swiss 3T3 cells.The Biochemical journal · 1994Article
- The zinc chelator 1,10-phenanthroline enhances the stimulatory effects of protein kinase C activators and staurosporine, but not sphingosine and H2O2, on phospholipase D activity in NIH 3T3 fibroblasts.The Biochemical journal · 1994Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A method for the rapid and quantitative separation of glycerophosphocholine, choline phosphate and choline upon ion-exchange columns is described. The method has been utilized to examine the stimulation of phosphatidylcholine breakdown in quiescent Swiss 3T3 cells in response to bombesin and 12-O-tetradecanoylphorbol 13-acetate (TPA). The stimulated generation of choline is shown to precede that of choline phosphate, with no effect upon glycerophosphocholine levels; but was attenuated in cells in which protein kinase C activity was down-regulated. The results thus suggest that stimulation of the cells with either bombesin or TPA activates phospholipase D-catalysed phosphatidylcholine breakdown by a common mechanism involving the activation of protein kinase C.
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