Evidence map›Paper›PMID 25970801›Full record

ArticleObesity (Silver Spring, Md.)2015

Reduced efficiency of sarcolipin-dependent respiration in myocytes from humans with severe obesity.

Christopher W Paran, Anthony R P Verkerke, Timothy D Heden, Sanghee Park, Kai Zou, Heather A Lawson, Haowei Song, John Turk, Joseph A Houmard, Katsuhiko Funai

Open access · bronzeAbstract readComparative Study
In one paragraph

Article in Obesity (Silver Spring, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 47 citations in OpenAlex.

  1. Effects of electromyostimulation on sarcolipin and CaBiochemistry and biophysics reports · 2026
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  10. Hypermetabolism in mice carrying a near complete human chromosome 21.bioRxiv : the preprint server for biology · 2023
    Article
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  12. Altered skeletal muscle sarco-endoplasmic reticulum CaAmerican journal of physiology. Regulatory, integrative and comparative physiology · 2022
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  15. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Christopher W ParanEast Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA.
Anthony R P VerkerkeEast Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA.
Timothy D HedenEast Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA.
Sanghee ParkEast Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA.
Kai ZouEast Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA.
Heather A LawsonDepartment of Genetics, Washington University School of Medicine, St. Louis, Missouri, USA.
Haowei SongMedicine Mass Spectrometry Facility, Washington University School of Medicine, St. Louis, Missouri, USA.
John TurkMedicine Mass Spectrometry Facility, Washington University School of Medicine, St. Louis, Missouri, USA.
Joseph A HoumardEast Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA.
Katsuhiko FunaiEast Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA.
East Carolina University · USWashington University in St. Louis · US

Funding

Washington University Nutrition Obesity Research CenterP30DK056341 · NIDDK · WASHINGTON UNIVERSITY · PI Jonathan R Brestoff · 1999 to 2026
$30.2M
WU P&FP30DK020579 · NIDDK · WASHINGTON UNIVERSITY · PI Clay F. Semenkovich · 2013 to 2026
$27.1M
TRANSGENICS COREP60DK020579 · NIDDK · WASHINGTON UNIVERSITY · PI SCHAFFER, JEAN E. · 1986 to 2012
$25.2M
TrainingP41GM103422 · NIGMS · WASHINGTON UNIVERSITY · PI YARASHESKI, KEVIN E · 2012 to 2019
$12.0M
LIPID METABOLISM IN OBESITY, WEIGHT LOSS AND EXERCISER01DK056112 · NIDDK · EAST CAROLINA UNIVERSITY · PI HOUMARD, JOSEPH A · 2000 to 2016
$4.5M
SKELETAL MUSCLE DE NOVO LIPOGENESIS AND THE GLUCOSE-FATTY ACID CYCLEK01DK095774 · NIDDK · WASHINGTON UNIVERSITY · PI FUNAI, KATSUHIKO · 2013 to 2016
$512k
IDENTIFYING SIGNATURES OF IMPRINTING EFFECTS ASSOCIATED WITH OBESITY AND T2DK01DK095003 · NIDDK · WASHINGTON UNIVERSITY · PI LAWSON, HEATHER A. · 2013 to 2016
$492k
NIDDK NIH HHS DK056112NIDDK NIH HHS DK095003NIDDK NIH HHS DK095774NIDDK NIH HHS K01 DK095003NIDDK NIH HHS K01 DK095774NIDDK NIH HHS P30 DK020579NIDDK NIH HHS P30 DK056341NIDDK NIH HHS P30-DK56341NIDDK NIH HHS P60 DK020579NIDDK NIH HHS P60-DK20579NIDDK NIH HHS R01 DK056112NIGMS NIH HHS P41 GM103422NIGMS NIH HHS P41-GM103422
6 · The paper itself

Abstract

objectiveSarcolipin (SLN) regulates muscle energy expenditure through its action on sarco/endoplasmic reticulum Ca(2+) -ATPase (SERCA) pump. It is unknown whether SLN-dependent respiration has relevance to human obesity, but whole-transcriptome gene expression profiling revealed that SLN was more highly expressed in myocytes from individuals with severe obesity (OB) than in lean controls (LN). The purpose of this study was to examine SLN-dependent cellular respiratory rates in LN and OB human muscles.

methodsPrimary myocytes were isolated from muscle biopsy from seven LN and OB Caucasian females. Cellular respiration was assessed with and without lentivirus-mediated SLN knockdown in LN and OB myocytes.

resultsSLN mRNA and protein abundance was greater in OB compared to LN cells. Despite elevated SLN levels in wild-type OB cells, respiratory rates among SLN-deficient cells were higher in OB compared to LN. Obesity-induced reduction in efficiency of SLN-dependent respiration was associated with altered sarcoplasmic reticulum phospholipidome.

conclusionsSLN-dependent respiration is reduced in muscles from humans with severe obesity compared to lean controls. Identification of the molecular mechanism that affects SLN efficiency might lead to interventions that promote an increase in skeletal muscle energy expenditure.

Indexed as

Energy MetabolismFemaleHumansMuscle CellsMuscle ProteinsMuscle, SkeletalObesity, MorbidProteolipidsSarcoplasmic ReticulumSarcoplasmic Reticulum Calcium-Transporting ATPasesMuscle ProteinsProteolipidssarcolipinSarcoplasmic Reticulum Calcium-Transporting ATPases

Identifiers

PMID25970801
PMCPMC4483165
OpenAlexW2148979483

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.