ArticlePLoS neglected tropical diseases2015
Deep Sequencing Analysis of the Ixodes ricinus Haemocytome.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
- Activation of the tick Toll pathway to control infection of Ixodes ricinus by the apicomplexan parasite Babesia microti.PLoS pathogens · 2024Article
- Involvement of a Microplusin-like Gene (HlonML-1) in the Olfactory Chemosensation ofMicroorganisms · 2024Article
- The immune factors involved in the rapid clearance of bacteria from the midgut of the tickFrontiers in cellular and infection microbiology · 2024Article
- A longitudinal transcriptomic analysis from unfed to post-engorgement midguts of adult female Ixodes scapularis.Scientific reports · 2023Review
- Article
- Serpins in Tick Physiology and Tick-Host Interaction.Frontiers in cellular and infection microbiology · 2022Review
- What do we know about the microbiome ofFrontiers in cellular and infection microbiology · 2022Review
- Acquired tick resistance: The trail is hot.Parasite immunology · 2021Review
- Interactions Between Ticks and Lyme Disease Spirochetes.Current issues in molecular biology · 2021Article
- Tick Immune System: What Is Known, the Interconnections, the Gaps, and the Challenges.Frontiers in immunology · 2021Review
- Ticks: More Than Just a Pathogen Delivery Service.Frontiers in cellular and infection microbiology · 2021Article
- A physiologic overview of the organ-specific transcriptome of the cattle tick Rhipicephalus microplus.Scientific reports · 2020Article
- Catalogue of stage-specific transcripts in Ixodes ricinus and their potential functions during the tick life-cycle.Parasites & vectors · 2020Article
- Whole body transcriptomes and new insights into the biology of the tick Ixodes ricinus.Parasites & vectors · 2018Article
- Review
- Modulation of the tick gut milieu by a secreted tick protein favors Borrelia burgdorferi colonization.Nature communications · 2017Article
- In silico target network analysis of de novo-discovered, tick saliva-specific microRNAs reveals important combinatorial effects in their interference with vertebrate host physiology.RNA (New York, N.Y.) · 2017Article
- Gene Duplication and Protein Evolution in Tick-Host Interactions.Frontiers in cellular and infection microbiology · 2017Review
- DecipheringFrontiers in cellular and infection microbiology · 2017Review
- Analysis of the Salivary Gland Transcriptome of Unfed and Partially FedFrontiers in cellular and infection microbiology · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundIxodes ricinus is the main tick vector of the microbes that cause Lyme disease and tick-borne encephalitis in Europe. Pathogens transmitted by ticks have to overcome innate immunity barriers present in tick tissues, including midgut, salivary glands epithelia and the hemocoel. Molecularly, invertebrate immunity is initiated when pathogen recognition molecules trigger serum or cellular signalling cascades leading to the production of antimicrobials, pathogen opsonization and phagocytosis. We presently aimed at identifying hemocyte transcripts from semi-engorged female I. ricinus ticks by mass sequencing a hemocyte cDNA library and annotating immune-related transcripts based on their hemocyte abundance as well as their ubiquitous distribution. METHODOLOGY/PRINCIPAL
findingsDe novo assembly of 926,596 pyrosequence reads plus 49,328,982 Illumina reads (148 nt length) from a hemocyte library, together with over 189 million Illumina reads from salivary gland and midgut libraries, generated 15,716 extracted coding sequences (CDS); these are displayed in an annotated hyperlinked spreadsheet format. Read mapping allowed the identification and annotation of tissue-enriched transcripts. A total of 327 transcripts were found significantly over expressed in the hemocyte libraries, including those coding for scavenger receptors, antimicrobial peptides, pathogen recognition proteins, proteases and protease inhibitors. Vitellogenin and lipid metabolism transcription enrichment suggests fat body components. We additionally annotated ubiquitously distributed transcripts associated with immune function, including immune-associated signal transduction proteins and transcription factors, including the STAT transcription factor. CONCLUSIONS/SIGNIFICANCE: This is the first systems biology approach to describe the genes expressed in the haemocytes of this neglected disease vector. A total of 2,860 coding sequences were deposited to GenBank, increasing to 27,547 the number so far deposited by our previous transcriptome studies that serves as a discovery platform for studies with I. ricinus biochemistry and physiology.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.