ArticlePloS one2015
A de novo floral transcriptome reveals clues into Phalaenopsis orchid flower development.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Orchid genome evolution and trait innovation.Journal of integrative plant biology · 2026Review
- Modular control of orchid beauty: co-expression networks orchestrate organ development and evolution in Phalaenopsis flower.Plant molecular biology · 2026Article
- Molecular mechanisms underlying floral trait formation inHorticulture research · 2026Article
- Breeding of ornamental orchids with focus on Phalaenopsis: current approaches, tools, and challenges for this century.Heredity · 2024Review
- Transcriptomic Analysis Suggests Auxin Regulation in Dorsal-Ventral Petal Asymmetry of Wild ProgenitorInternational journal of molecular sciences · 2022Article
- Whole-transcriptome analysis of differentially expressed genes between ray and disc florets and identification of flowering regulatory genes inFrontiers in plant science · 2022Article
- Extending the Toolkit for Beauty: Differential Co-Expression ofInternational journal of molecular sciences · 2021Article
- Proteomic Analysis of the Early Development of theMolecules (Basel, Switzerland) · 2020Article
- Comparative Transcriptome Reveals the Genes' Adaption to Herkogamy ofFrontiers in genetics · 2020Article
- PeERF1, a SHINE-Like Transcription Factor, Is Involved in Nanoridge Development on Lip Epidermis ofFrontiers in plant science · 2019Article
- Comparative transcriptomics provides insight into the molecular basis of species diversification of section Trigonopedia (Cypripedium) on the Qinghai-Tibetan Plateau.Scientific reports · 2018Article
- Post genomics era for orchid research.Botanical studies · 2017Review
- Tissue-Specific Floral Transcriptome Analysis of the Sexually Deceptive OrchidFrontiers in plant science · 2017Article
- De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris.Scientific data · 2016Article
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Authors and funding
9 authors.
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Abstract
Phalaenopsis has a zygomorphic floral structure, including three outer tepals, two lateral inner tepals and a highly modified inner median tepal called labellum or lip; however, the regulation of its organ development remains unelucidated. We generated RNA-seq reads with the Illumina platform for floral organs of the Phalaenopsis wild-type and peloric mutant with a lip-like petal. A total of 43,552 contigs were obtained after de novo assembly. We used differentially expressed gene profiling to compare the transcriptional changes in floral organs for both the wild-type and peloric mutant. Pair-wise comparison of sepals, petals and labellum between peloric mutant and its wild-type revealed 1,838, 758 and 1,147 contigs, respectively, with significant differential expression. PhAGL6a (CUFF.17763), PhAGL6b (CUFF.17763.1), PhMADS1 (CUFF.36625.1), PhMADS4 (CUFF.25909) and PhMADS5 (CUFF.39479.1) were significantly upregulated in the lip-like petal of the peloric mutant. We used real-time PCR analysis of lip-like petals, lip-like sepals and the big lip of peloric mutants to confirm the five genes' expression patterns. PhAGL6a, PhAGL6b and PhMADS4 were strongly expressed in the labellum and significantly upregulated in lip-like petals and lip-like sepals of peloric-mutant flowers. In addition, PhAGL6b was significantly downregulated in the labellum of the big lip mutant, with no change in expression of PhAGL6a. We provide a comprehensive transcript profile and functional analysis of Phalaenopsis floral organs. PhAGL6a PhAGL6b, and PhMADS4 might play crucial roles in the development of the labellum in Phalaenopsis. Our study provides new insights into how the orchid labellum differs and why the petal or sepal converts to a labellum in Phalaenopsis floral mutants.
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