ReviewISRN biochemistry2013
Locus-specific biochemical epigenetics/chromatin biochemistry by insertional chromatin immunoprecipitation.
Review in ISRN biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Review
- Toehold-enhanced LNA probes for selective pull down and single-molecule analysis of native chromatin.Scientific reports · 2017Article
- Locus-specific ChIP combined with NGS analysis reveals genomic regulatory regions that physically interact with the Pax5 promoter in a chicken B cell line.DNA research : an international journal for rapid publication of reports on genes and genomes · 2017Article
- Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP.Journal of visualized experiments : JoVE · 2016Article
- Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies.Gene regulation and systems biology · 2016Review
- Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research.International journal of molecular sciences · 2015Review
- Isolation of Specific Genomic Regions and Identification of Their Associated Molecules by Engineered DNA-Binding Molecule-Mediated Chromatin Immunoprecipitation (enChIP) Using the CRISPR System and TAL Proteins.International journal of molecular sciences · 2015Review
- Identification of non-coding RNAs associated with telomeres using a combination of enChIP and RNA sequencing.PloS one · 2015Article
- A critical role of the Thy28-MYH9 axis in B cell-specific expression of the Pax5 gene in chicken B cells.PloS one · 2015Article
- Efficient isolation of specific genomic regions retaining molecular interactions by the iChIP system using recombinant exogenous DNA-binding proteins.BMC molecular biology · 2014Article
- Identification of proteins associated with an IFNγ-responsive promoter by a retroviral expression system for enChIP using CRISPR.PloS one · 2014Article
- Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP).Scientific reports · 2013Article
- Combining genomic and proteomic approaches for epigenetics research.Epigenomics · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comprehensive understanding of regulation mechanisms of biological phenomena mediated by functions of genomic DNA requires identification of molecules bound to genomic regions of interest in vivo. However, nonbiased methods to identify molecules bound to specific genomic loci in vivo are limited. To perform biochemical and molecular biological analysis of specific genomic regions, we developed the insertional chromatin immunoprecipitation (iChIP) technology to purify the genomic regions of interest. We applied iChIP to direct identification of components of insulator complexes, which function as boundaries of chromatin domain, showing that it is feasible to directly identify proteins and RNA bound to a specific genomic region in vivo by using iChIP. In addition, recently, we succeeded in identifying proteins and genomic regions interacting with a single copy endogenous locus. In this paper, we will discuss the application of iChIP to epigenetics and chromatin research.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.