ArticleThe Journal of biological chemistry2015
Members of the Plant CRK Superfamily Are Capable of Trans- and Autophosphorylation of Tyrosine Residues.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 54 citations in OpenAlex.
- Genome-Wide Investigation ofInternational journal of molecular sciences · 2025Article
- Intricate intracellular kinase network regulates the Spodoptera lituta-derived elicitor response signaling in Arabidopsis.Journal of plant research · 2025Article
- Applications of the wheat germ cell-free protein synthesis system in plant biochemical studies.Plant biotechnology (Tokyo, Japan) · 2024Article
- Emerging Functions of Protein Tyrosine Phosphatases in Plants.International journal of molecular sciences · 2024Review
- DNA-encoded probe-based assay for profiling plant kinase activities.PNAS nexus · 2024Article
- Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The putative myristoylome of Physcomitrium patens reveals conserved features of myristoylation in basal land plants.Plant cell reports · 2023Article
- Cytoplasmic Kinase Network Mediates Defense Response toPlants (Basel, Switzerland) · 2023Article
- Importance of Tyrosine Phosphorylation in Hormone-Regulated Plant Growth and Development.International journal of molecular sciences · 2022Review
- Plant Kinases in the Perception and Signaling Networks Associated With Arthropod Herbivory.Frontiers in plant science · 2022Review
- Tissue-specific expression pattern of calcium-dependent protein kinases-related kinases (CRKs) in rice.Plant signaling & behavior · 2020Article
- Characterization of Atypical Protein Tyrosine Kinase (PTK) Genes and Their Role in Abiotic Stress Response in Rice.Plants (Basel, Switzerland) · 2020Article
- d-Lactic acid secreted by Chlorella fusca primes pattern-triggered immunity against Pseudomonas syringae in Arabidopsis.The Plant journal : for cell and molecular biology · 2020Article
- Evidence that ERF transcriptional regulators serve as possible key molecules for natural variation in defense against herbivores in tall goldenrod.Scientific reports · 2020Article
- Calcium-Regulated Phosphorylation Systems Controlling Uptake and Balance of Plant Nutrients.Frontiers in plant science · 2020Review
- H2Bub1 RegulatesPlant physiology · 2020Article
- Computational Disorder Analysis in Ethylene Response Factors Uncovers Binding Motifs Critical to Their Diverse Functions.International journal of molecular sciences · 2019Article
- AtCRK5 Protein Kinase Exhibits a Regulatory Role in Hypocotyl Hook Development during Skotomorphogenesis.International journal of molecular sciences · 2019Article
- The Recruitment Model of Metabolic Evolution: Jasmonate-Responsive Transcription Factors and a Conceptual Model for the Evolution of Metabolic Pathways.Frontiers in plant science · 2019Review
- Tyrosine Kinase-Dependent Defense Responses Against Herbivory in Arabidopsis.Frontiers in plant science · 2019Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein phosphorylation on Tyr residues is a key post-translational modification in mammals. In plants, recent studies have identified Tyr-specific protein phosphatase and Tyr-phosphorylated proteins in Arabidopsis by phosphoproteomic screenings, implying that plants have a Tyr phosphorylation signal pathway. However, little is known about the protein kinases (PKs) involved in Tyr phosphorylation in plants. Here, we demonstrate that Arabidopsis calcium-dependent protein kinase (CDPK/CPK)-related PKs (CRKs) have high Tyr-autophosphorylation activity and that they can phosphorylate Tyr residue(s) on substrate proteins in Arabidopsis. To identify PKs for Tyr phosphorylation, we examined the autophosphorylation activity of 759 PKs using an Arabidopsis protein array based on a wheat cell-free system. In total, we identified 38 PKs with Tyr-autophosphorylation activity. The CRK family was a major protein family identified. A cell-free substrate screening revealed that these CRKs phosphorylate β-tubulin (TBB) 2, TBB7, and certain transcription factors (TFs) such as ethylene response factor 13 (ERF13). All five CRKs tested showed Tyr-auto/trans-phosphorylation activity and especially two CRKs, CRK2 and CRK3, showed a high ERF13 Tyr-phosphorylation activity. A cell-based transient expression assay revealed that Tyr(16/)Tyr(207) sites in ERF13 were phosphorylated by CRK3 and that Tyr phosphorylation of endogenous TBBs occurs in CRK2 overexpressing cells. Furthermore, crk2 and crk3 mutants showed a decrease in the Tyr phosphorylation level of TBBs. These results suggest that CRKs have Tyr kinase activity, and these might be one of the major PKs responsible for protein Tyr phosphorylation in Arabidopsis plants.
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