ArticleThe Journal of physiology2015
Voltage-gated sodium channels contribute to action potentials and spontaneous contractility in isolated human lymphatic vessels.
Article in The Journal of physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
- Pacemaking in the lymphatic system.The Journal of physiology · 2026Review
- Guidelines for evaluating endothelial function in vascular tissue.American journal of physiology. Heart and circulatory physiology · 2026Review
- The Thoracic Lymphatic Vasculature: Interorgan Strategies to Achieve an Optimal Tissue Function.Comprehensive Physiology · 2026Review
- Single-Cell Transcriptomics and Lineage Tracing Unveil Parallels in Lymphatic Muscle and Venous Smooth Muscle Development, Identity, and Function.Arteriosclerosis, thrombosis, and vascular biology · 2025Article
- Aging-induced changes in lymphatic muscle cell transcriptomes are associated with reduced pumping of peripheral collecting lymphatic vessels in mice.Developmental cell · 2025Article
- Veratridine-Induced Oscillations in Nav 1.7 but Not Nav 1.5 Sodium Channels Are Revealed by Membrane Potential Sensitive Dye.Membranes · 2025Article
- Modelling pacemaker oscillations in lymphatic muscle cells: lengthened action potentials by two distinct system effects.Royal Society open science · 2025Article
- Overview of Lymphatic Muscle Cells in Development, Physiology, and Disease.Microcirculation (New York, N.Y. : 1994) · 2024Review
- Dynamic multispectral NIR/SWIR forJournal of biomedical optics · 2024Article
- Transcriptional, developmental, and functional parallels of lymphatic and venous smooth muscle.bioRxiv : the preprint server for biology · 2024Article
- Electric field stimulation unmasks a subtle role for T-type calcium channels in regulating lymphatic contraction.Scientific reports · 2023Article
- ERG KScientific reports · 2023Article
- Electric field stimulation unmasks a subtle role for T-type calcium channels in regulating lymphatic contraction.Research square · 2023Article
- Vascular mechanotransduction.Physiological reviews · 2023Review
- Lymphatic Clearance and Pump Function.Cold Spring Harbor perspectives in medicine · 2023Review
- Review
- KAmerican journal of physiology. Cell physiology · 2022Review
- Positive chronotropic action of HCN channel antagonism in human collecting lymphatic vessels.Physiological reports · 2022Article
- Drug-Related Lymphedema: Mysteries, Mechanisms, and Potential Therapies.Frontiers in pharmacology · 2022Review
- Diagnosis and Management of Lymphatic Disorders in Congenital Heart Disease.Current cardiology reports · 2020Review
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Voltage-gated sodium channels (VGSC) play a key role for initiating action potentials (AP) in excitable cells. VGSC in human lymphatic vessels have not been investigated. In the present study, we report the electrical activity and APs of small human lymphatic collecting vessels, as well as mRNA expression and function of VGSC in small and large human lymphatic vessels. The VGSC blocker TTX inhibited spontaneous contractions in six of 10 spontaneously active vessels, whereas ranolazine, which has a narrower VGSC blocking profile, had no influence on spontaneous activity. TTX did not affect noradrenaline-induced contractions. The VGSC opener veratridine induced contractions in a concentration-dependent manner (0.1-30 μm) eliciting a stable tonic contraction and membrane depolarization to -18 ± 0.6 mV. Veratridine-induced depolarizations and contractions were reversed ∼80% by TTX, and were dependent on Ca(2+) influx via L-type calcium channels and the sodium-calcium exchanger in reverse mode. Molecular analysis determined NaV 1.3 to be the predominantly expressed VGSC isoform. Electrophysiology of mesenteric lymphatics determined the resting membrane potential to be -45 ± 1.7 mV. Spontaneous APs were preceded by a slow depolarization of 5.3 ± 0.6 mV after which a spike was elicited that almost completely repolarized before immediately depolarizing again to plateau. Vessels transiently hyperpolarized prior to returning to the resting membrane potential. TTX application blocked APs. We have shown that VGSC are necessary for initiating and maintaining APs and spontaneous contractions in human lymphatic vessels and our data suggest the main contribution from comes NaV 1.3. We have also shown that activation of these channels augments the contractile activity of the vessels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.