ArticleProtoplasma2016
Toward establishing a morphological and ultrastructural characterization of proembryogenic masses and early somatic embryos of Araucaria angustifolia (Bert.) O. Kuntze.
Article in Protoplasma, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 41 citations in OpenAlex.
- Somatic Embryogenesis in Conifers: One Clade to Rule Them All?Plants (Basel, Switzerland) · 2023Review
- Reactive oxygen metabolism in the proliferation of Korean pine embryogenic callus cells promoted by exogenous GSH.Scientific reports · 2023Article
- Exogenous Glutathione Promotes the Proliferation ofPlants (Basel, Switzerland) · 2022Article
- Updating embryonic ontogenesis in Araucaria angustifolia: from Burlingame (1915) to the present.Protoplasma · 2020Article
- Structural aspects of cypsela and seed development of Trichocline catharinensis (Cabrera): a Brazilian endemic species.Protoplasma · 2019Article
- Effect of Thermal Stress on Tissue Ultrastructure and Metabolite Profiles During Initiation of Radiata Pine Somatic Embryogenesis.Frontiers in plant science · 2018Article
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Corrections and comments
- Erratum issuedErratum to: Toward establishing a morphological and ultrastructural characterization of proembryogenic masses and early somatic embryos of Araucaria angustifolia (Bert.) O. Kuntze.2017 · Schmidt, Daniela [corrected to Schmitz, Daniela]
Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Somatic embryogenesis is a morphogenetic route useful for the study of embryonic development, as well as the large-scale propagation of endangered species, such as the Brazilian pine (Araucaria angustifolia). In the present study, we investigated the morphological and ultrastructural organization of A. angustifolia somatic embryo development by means of optical and electron microscopy. The proembryogenic stage was characterized by the proliferation of proembryogenic masses (PEMs), which are cellular aggregates composed of embryogenic cells (ECs) attached to suspensor-like cells (SCs). PEMs proliferate through three developmental stages, PEM I, II, and III, by changes in the number of ECs and SCs. PEM III-to-early somatic embryo (SE) transition was characterized by compact clusters of ECs growing out of PEM III, albeit still connected to it by SCs. Early SEs showed a dense globular embryonic mass (EM) and suspensor region (SR) connected by embryonic tube cells (TCs). By comparison, early somatic and zygotic embryos showed similar morphology. ECs are round with a large nucleus, nucleoli, and many cytoplasmic organelles. In contrast, TCs and SCs are elongated and vacuolated with cellular dismantling which is associated with programmed cell death of SCs. Abundant starch grains were observed in the TCs and SCs, while proteins were more abundant in the ECs. Based on the results of this study, a fate map of SE development in A. angustifolia is, for the first time, proposed. Additionally, this study shows the cell biology of SE development of this primitive gymnosperm which may be useful in evolutionary studies in this area.
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