ArticleDevelopment (Cambridge, England)2015
CSR-1 and P granules suppress sperm-specific transcription in the C. elegans germline.
Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
54 citing papers in PubMed, 78 citations in OpenAlex.
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- Remodeling of Germ Cell mRNPs for Translational Control.Biology · 2025Review
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- Intrinsically disordered arginine-glycine repeat domains tune sub-nucleolar compartmentalization in Caenorhabditis elegans.Genetics · 2025Article
- WAGO-1 is a sexually dimorphic Argonaute protein required for proper germ granule structure and gametogenesis.bioRxiv : the preprint server for biology · 2025Article
- RNA Helicase A promotes small RNA biogenesis and sorting in germ cells.bioRxiv : the preprint server for biology · 2025Article
- Small RNA-mediated genetic switches coordinate ALG-3/4 small RNA pathway function.Nucleic acids research · 2024Article
- RNAi-dependent expression of sperm genes in ADL chemosensory neurons is required for olfactory responses inFrontiers in molecular biosciences · 2024Article
- Inheritance of Stress Responses via Small Non-Coding RNAs in Invertebrates and Mammals.Epigenomes · 2023Review
- C. elegans germ granules sculpt both germline and somatic RNAome.Nature communications · 2023Article
- A small RNA system ensures accurate homologous pairing and unpaired silencing of meiotic chromosomes.The EMBO journal · 2023Article
- Transcriptome-wide analyses of piRNA binding sites suggest distinct mechanisms regulate piRNA binding and silencing inRNA (New York, N.Y.) · 2023Article
- piRNAs regulate a Hedgehog germline-to-soma pro-aging signal.Nature aging · 2023Article
- GLH-1/Vasa represses neuropeptide expression and drives spermiogenesis in the C. elegans germline.Developmental biology · 2022Article
- RG/RGG repeats in the C. elegans homologs of Nucleolin and GAR1 contribute to sub-nucleolar phase separation.Nature communications · 2022Article
- Target-dependent suppression of siRNA production modulates the levels of endogenous siRNAs in the Caenorhabditis elegans germline.Development (Cambridge, England) · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Germ granules (P granules) in C. elegans are required for fertility and function to maintain germ cell identity and pluripotency. Sterility in the absence of P granules is often accompanied by the misexpression of soma-specific proteins and the initiation of somatic differentiation in germ cells. To investigate whether this is caused by the accumulation of somatic transcripts, we performed mRNA-seq on dissected germlines with and without P granules. Strikingly, we found that somatic transcripts do not increase in the young adult germline when P granules are impaired. Instead, we found that impairing P granules causes sperm-specific mRNAs to become highly overexpressed. This includes the accumulation of major sperm protein (MSP) transcripts in germ cells, a phenotype that is suppressed by feminization of the germline. A core component of P granules, the endo-siRNA-binding Argonaute protein CSR-1, has recently been ascribed with the ability to license transcripts for germline expression. However, impairing CSR-1 has very little effect on the accumulation of its mRNA targets. Instead, we found that CSR-1 functions with P granules to prevent MSP and sperm-specific mRNAs from being transcribed in the hermaphrodite germline. These findings suggest that P granules protect germline integrity through two different mechanisms, by (1) preventing the inappropriate expression of somatic proteins at the level of translational regulation, and by (2) functioning with CSR-1 to limit the domain of sperm-specific expression at the level of transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.