ArticleJournal of immunological methods2015
An improved method for differentiating cell-bound from internalized particles by imaging flow cytometry.
Article in Journal of immunological methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- Force Spectroscopy by Atomic Force Microscopy as Indicator for Cellular Microplastic Uptake.International journal of molecular sciences · 2026Article
- Uptake of Micro- and Nano-Sized Plastic Particles in Human Cells: Size-Dependent Endocytosis and Cellular Responses.BioMed research international · 2026Article
- Translation of the Morphological Hallmarks of Dyserythropoiesis to Objective Morphometric Parameters by Imaging Flow Cytometry.International journal of laboratory hematology · 2025Article
- Distinct neutrophil effector functions in response to different isolates of Leishmania aethiopica.Parasites & vectors · 2024Article
- Cytometry in High-Containment Laboratories.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Phagocytosis via complement receptor 3 enables microbes to evade killing by neutrophils.Journal of leukocyte biology · 2023Article
- A Real-Time Image-Based Efferocytosis Assay for the Discovery of Functionally Inhibitory Anti-MerTK Antibodies.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- Label-Free Identification of Persistent Particles in Association with Primary Immune Cells by Imaging Flow Cytometry.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lungFrontiers in toxicology · 2023Review
- Variable Expression of Opa Proteins by Neisseria gonorrhoeae Influences Bacterial Association and Phagocytic Killing by Human Neutrophils.Journal of bacteriology · 2022Article
- Use of Flow Cytometry to Evaluate Phagocytosis ofFrontiers in immunology · 2021Article
- Imaging Flow Cytometry and Confocal Immunofluorescence Microscopy of Virus-Host Cell Interactions.Frontiers in cellular and infection microbiology · 2021Review
- Extracellular adenosine signaling reverses the age-driven decline in the ability of neutrophils to kill Streptococcus pneumoniae.Aging cell · 2020Article
- Human Neutrophil Granule Exocytosis in Response toPathogens (Basel, Switzerland) · 2020Article
- Using Imaging Flow Cytometry to Quantify Neutrophil Phagocytosis.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Imaging flow cytometry methods for quantitative analysis of label-free crystalline silica particle interactions with immune cells.AIMS biophysics · 2020Article
- Imaging Flow Cytometry Protocols for Examining Phagocytosis of Microplastics and Bioparticles by Immune Cells of Aquatic Animals.Frontiers in immunology · 2020Article
- Protocols to Interrogate the Interactions Between Neisseria gonorrhoeae and Primary Human Neutrophils.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Human immunodeficiency virus type-1 (HIV-1) evades antibody-dependent phagocytosis.PLoS pathogens · 2017Article
- Imaging flow cytometry assays for quantifying pigment grade titanium dioxide particle internalization and interactions with immune cells in whole blood.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Recognition, binding, internalization, and elimination of pathogens and cell debris are important functions of professional as well as non-professional phagocytes. However, high-throughput methods for quantifying cell-associated particles and discriminating bound from internalized particles have been lacking. Here we describe a protocol for using imaging flow cytometry to quantify the attached and phagocytosed particles that are associated with a population of cells. Cells were exposed to fluorescent particles, fixed, and exposed to an antibody of a different fluorophore that recognizes the particles. The antibody is added without cell permeabilization, such that the antibody only binds extracellular particles. Cells with and without associated particles were identified by imaging flow cytometry. For each cell with associated particles, a spot count algorithm was employed to quantify the number of extracellular (double fluorescent) and intracellular (single fluorescent) particles per cell, from which the percent particle internalization was determined. The spot count algorithm was empirically validated by examining the fluorescence and phase contrast images acquired by the flow cytometer. We used this protocol to measure binding and internalization of the bacterium Neisseria gonorrhoeae by primary human neutrophils, using different bacterial variants and under different cellular conditions. The results acquired using imaging flow cytometry agreed with findings that were previously obtained using conventional immunofluorescence microscopy. This protocol provides a rapid, powerful method for measuring the association and internalization of any particle by any cell type.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.