ArticleScientific reports2015
Phase-contrast zoom tomography reveals precise locations of macrophages in mouse lungs.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 79 citations in OpenAlex.
- For your eyes too: a new set of images of biological materials from the scientific commissioning of the MOGNO beamline at Sirius.Journal of synchrotron radiation · 2026Article
- Synchrotron X-ray imaging of soft biological tissues - principles, applications and future prospects.Journal of cell science · 2024Review
- In situ label-free X-ray imaging for visualizing the localization of nanomedicines and subcellular architecture in intact single cells.Nature protocols · 2024Review
- Neodymium acetate as a contrast agent for X-ray phase-contrast tomography.Journal of medical imaging (Bellingham, Wash.) · 2023Article
- Accurate real space iterative reconstruction (RESIRE) algorithm for tomography.Scientific reports · 2023Article
- Hierarchically guided in situ nanolaminography for the visualisation of damage nucleation in alloy sheets.Scientific reports · 2023Article
- Advanced Light Source Analytical Techniques for Exploring the Biological Behavior and Fate of Nanomedicines.ACS central science · 2022Review
- Synchrotron tomography applications in agriculture and food sciences research: a review.Plant methods · 2022Review
- In-situ x-ray fluorescence imaging of the endogenous iodine distribution in murine thyroids.Scientific reports · 2022Article
- Microscopic Visualization of Cell-Cell Adhesion Complexes at Micro and Nanoscale.Frontiers in cell and developmental biology · 2022Review
- Imaging intact human organs with local resolution of cellular structures using hierarchical phase-contrast tomography.Nature methods · 2021Article
- X-ray-Based Techniques to Study the Nano-Bio Interface.ACS nano · 2021Article
- X-Ray Structural Analysis of Single Adult Cardiomyocytes: Tomographic Imaging and Microdiffraction.Biophysical journal · 2020Article
- Fiber orientation in a whole mouse heart reconstructed by laboratory phase-contrast micro-CT.Journal of medical imaging (Bellingham, Wash.) · 2020Article
- X-ray computed tomography in life sciences.BMC biology · 2020Review
- Imaging cell morphology and physiology using X-rays.Biochemical Society transactions · 2019Review
- X-ray-Based 3D Virtual Histology-Adding the Next Dimension to Histological Analysis.Molecular imaging and biology · 2018Review
- A quantitative analysis of 3D-cell distribution in regenerating muscle-skeletal system with synchrotron X-ray computed microtomography.Scientific reports · 2018Article
- Correlative microscopy approach for biology using X-ray holography, X-ray scanning diffraction and STED microscopy.Nature communications · 2018Article
- X-ray computed tomography and its potential in ecological research: A review of studies and optimization of specimen preparation.Ecology and evolution · 2018Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have performed x-ray phase-contrast tomography on mouse lung tissue. Using a divergent x-ray beam generated by nanoscale focusing, we used zoom tomography to produce three-dimensional reconstructions with selectable magnification, resolution, and field of view. Thus, macroscopic tissue samples extending over several mm can be studied in sub-cellular-level structural detail. The zoom capability and, in particular, the high dose efficiency are enabled by the near-perfect exit wavefront of an optimized x-ray waveguide channel. In combination with suitable phase-retrieval algorithms, challenging radiation-sensitive and low-contrast samples can be reconstructed with minimal artefacts. The dose efficiency of the method is demonstrated by the reconstruction of living macrophages both with and without phagocytized contrast agents. We also used zoom tomography to visualize barium-labelled macrophages in the context of morphological structures in asthmatic and healthy mouse lung tissue one day after intratracheal application. The three-dimensional reconstructions showed that the macrophages predominantly localized to the alveoli, but they were also found in bronchial walls, indicating that these cells might be able to migrate from the lumen of the bronchi through the epithelium.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.