ArticlePloS one2015
Developmental Competence of Vitrified-Warmed Bovine Oocytes at the Germinal-Vesicle Stage is Improved by Cyclic Adenosine Monophosphate Modulators during In Vitro Maturation.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- Impact of Maturation and Vitrification Time of Human GV Oocytes on the Metaphase Plate Configuration.International journal of molecular sciences · 2021Trial
- Malonic acid promotes glycolytic activity in cumulus cells and improves the quality of bovine oocytes matured in vitro.The Journal of reproduction and development · 2026Article
- Disparity between Post-thaw Survival and Functional Integrity of Vitrified-thawed Oocytes from Obese Mice.JBRA assisted reproduction · 2026Article
- Effect of Pre-IVM Duration with cAMP Modulators on the Production of Cloned Equine Embryos and Foals.Animals : an open access journal from MDPI · 2025Article
- Supplementation of Forskolin and Linoleic Acid During IVC Improved the Developmental and Vitrification Efficiency of Bovine Embryos.International journal of molecular sciences · 2025Article
- Lack of effect of cyclic adenosine monophosphate modulation during prematuration in vitro on development to the blastocyst stage and expression of specific genes associated with lineage commitment.JDS communications · 2025Article
- New Strategies for Conservation of Gentile di Puglia Sheep Breed, an Autochthonous Capital of Millennial Tradition in Southern Italy.Animals : an open access journal from MDPI · 2023Article
- Genome transfer technique for bovine embryo production using the metaphase plate and polar body.Journal of assisted reproduction and genetics · 2023Article
- Multidrug resistance transporter-1 dysfunction perturbs meiosis and Ca2+ homeostasis in oocytes.Reproduction (Cambridge, England) · 2023Article
- Potential Development of Vitrified Immature Human Oocytes: Influence of the Culture Medium and the Timing of Vitrification.International journal of molecular sciences · 2022Article
- Effects of cyclic adenosine monophosphate modulators on maturation and quality of vitrified-warmed germinal vesicle stage mouse oocytes.Reproductive biology and endocrinology : RB&E · 2020Article
- Natural honey acts as a nonpermeating cryoprotectant for promoting bovine oocyte vitrification.PloS one · 2020Article
- Meiotic arrest with roscovitine and follicular fluid improves cytoplasmic maturation of porcine oocytes by promoting chromatin de-condensation and gene transcription.Scientific reports · 2017Article
- Adenosine Modulates the Oocyte Developmental Competence by Exposing Stages and Synthetic Blocking during In Vitro Maturation.Development & reproduction · 2016Article
- High survival of mouse oocytes using an optimized vitrification protocol.Scientific reports · 2016Article
- Cryodevices developed for minimum volume cooling vitrification of bovine oocytes.Animal science journal = Nihon chikusan GakkaihoReview
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryopreservation of mature oocytes and embryos has provided numerous benefits in reproductive medicine. Although successful cryopreservation of germinal-vesicle stage (GV) oocytes holds promise for further advances in reproductive biology and clinical embryology fields, reports regarding cryopreservation of immature oocytes are limited. Oocyte survival and maturation rates have improved since vitrification is being performed at the GV stage, but the subsequent developmental competence of GV oocytes is still low. The purpose of this study was to evaluate the effects of supplementation of the maturation medium with cyclic adenosine monophosphate (cAMP) modulators on the developmental competence of vitrified-warmed GV bovine oocytes. GV oocytes were vitrified-warmed and cultured to allow for oocyte maturation, and then parthenogenetically activated or fertilized in vitro. Our results indicate that addition of a cAMP modulator forskolin (FSK) or 3-isobutyl-1-methylxanthine (IBMX) to the maturation medium significantly improved the developmental competence of vitrified-warmed GV oocytes. We also demonstrated that vitrification of GV oocytes led to a decline in cAMP levels and maturation-promoting factor (MPF) activity in the oocytes during the initial and final phases of maturation, respectively. Nevertheless, the addition of FSK or IBMX to the maturation medium significantly elevated cAMP levels and MPF activity during IVM. Taken together, our results suggest that the cryopreservation-associated meiotic and developmental abnormalities observed in GV oocytes may be ameliorated by an artificial increase in cAMP levels during maturation culture after warming.
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