ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2015
Transcriptional profiling in pearl millet (Pennisetum glaucum L.R. Br.) for identification of differentially expressed drought responsive genes.
Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 2 of them syntheses that pooled it.
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Who cites it
23 citing papers in PubMed, 2 syntheses or guidelines pooled it, 48 citations in OpenAlex.
- Identification of responsive genes to multiple abiotic stresses in rice (Oryza sativa): a meta-analysis of transcriptomics data.Scientific reports · 2024Pooled it
- Pearl millet response to drought: A review.Frontiers in plant science · 2023Pooled it
- Comprehensive analysis of housekeeping genes, tissue-specific genes, and dynamic regulation across developmental stages in pearl millet.BMC genomics · 2024Article
- Article
- Genetic insights in pearl millet breeding in the genomic era: challenges and prospects.Plant biotechnology reports · 2023Review
- Transcriptome-based analysis of key pathways relating to yield formation stage of foxtail millet under different drought stress conditions.Frontiers in plant science · 2022Article
- Transcriptome profiling of two rice genotypes under mild field drought stress during grain-filling stage.AoB PLANTS · 2021Article
- Research on the drought tolerance mechanism of Pennisetum glaucum (L.) in the root during the seedling stage.BMC genomics · 2021Article
- Morpho-physiological and biochemical changes in finger millet [Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Comparative transcriptome analysis reveals the genes and pathways involved in terminal drought tolerance in pearl millet.Plant molecular biology · 2020Article
- Transcriptome analysis of heat stress and drought stress in pearl millet based on Pacbio full-length transcriptome sequencing.BMC plant biology · 2020Article
- Understanding the Genetic Basis of Spike Fertility to Improve Grain Number, Harvest Index, and Grain Yield in Wheat Under High Temperature Stress Environments.Frontiers in plant science · 2019Article
- Transcript profiling and gene expression analysis under drought stress in Ziziphus nummularia (Burm.f.) Wright & Arn.Molecular biology reports · 2018Article
- Transcriptomic signature of drought response in pearl millet (Pennisetum glaucum (L.) and development of web-genomic resources.Scientific reports · 2018Article
- Milestones achieved in response to drought stress through reverse genetic approaches.F1000Research · 2018Review
- Article
- Systems biology and genome-wide approaches to unveil the molecular players involved in the pre-germinative metabolism: implications on seed technology traits.Plant cell reports · 2017Review
- Cereal Crop Proteomics: Systemic Analysis of Crop Drought Stress Responses Towards Marker-Assisted Selection Breeding.Frontiers in plant science · 2017Review
- Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics.International journal of molecular sciences · 2016Review
- Exploration of Genetic and Genomic Resources for Abiotic and Biotic Stress Tolerance in Pearl Millet.Frontiers in plant science · 2016Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pearl millet (Pennisetum glaucum) is an important cereal of traditional farming systems that has the natural ability to withstand various abiotic stresses. The present study aims at the identification and validation of major differentially expressed genes in response to drought stress in P. glaucum by Suppression Subtractive Hybridization (SSH) analysis. Twenty-two days old seedlings of P. glaucum cultivar PPMI741 were subjected to drought stress by treatment of 30 % Polyethylene glycol for different time periods 30 min (T1), 2 h (T2), 4 h (T3), 8 h (T4), 16 h (T5), 24 h (T6) and 48 h (T7) respectively, monitored by examining the RWC of seedlings. Total RNA was isolated to construct drought responsive subtractive cDNA library through SSH, sequenced to identify the differentially expressed genes in response to drought stress and validated by qRT-PCR.745 ESTs were assembled into a collection of 299 unigenes having 52 contigs and 247 singletons. All 745 ESTs were submitted to ENA-EMBL databases (Accession no. HG516611- HG517355). After analysis, 10 differentially expressed genes were validated namely Abscisic stress ripening protein, Ascorbate peroxidase, Inosine-5'-monophosphate dehydrogenase, Putative beta-1, 3-glucanase, Glyoxalase, Rab7, Aspartic proteinase Oryzasin, DnaJ-like protein and Calmodulin-like protein by qRT-PCR. The identified ESTs reveal a major portion of the stress responsive transcriptome that may prove to be a vent to unravel molecular basis underlying tolerance of pearl millet (Pennisetum glaucum) to drought stress. These genes could be utilized for transgenic breeding or transferred to crop plants through marker assisted selection for the development of better drought resistant cultivars having enhanced adaptability to survive harsh environmental conditions.
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