ArticleProceedings of the National Academy of Sciences of the United States of America2015
Protein aggregation in salt solutions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it.
- BioMThermDB 1.0: Thermophysical Database of Proteins in Solutions.International journal of molecular sciences · 2022Pooled it
- Modeling Opposite Effects of an Additive on Liquid-Liquid Phase Separation and Crystal Solubility of Protein Solutions.Molecules (Basel, Switzerland) · 2026Article
- HAPLN2 forms aggregates and promotes microglial inflammation during brain aging in mice.PLoS biology · 2025Article
- Anionic Lipid Catalyzes the Generation of Cytotoxic Insulin Oligomers.Biomolecules · 2025Article
- Coomassie Brilliant Blue Induces Coiled-Coil Aggregation in Lysozyme at pH 7.4 by Hydrophobic and Electrostatic Forces.ACS omega · 2025Article
- Myofilamental integrity of the myometrium due to cold ischaemia stress during autotransplantation in the experimental sheep model.PloS one · 2025Article
- Deciphering Spectroscopic Signatures of Competing CaThe journal of physical chemistry. B · 2024Article
- Combining Scattering Experiments and Colloid Theory to Characterize Charge Effects in Concentrated Antibody Solutions.Molecular pharmaceutics · 2024Article
- A Unified Description of Salt Effects on the Liquid-Liquid Phase Separation of Proteins.ACS central science · 2024Article
- Multiscale Simulations to Discover Self-Assembled Oligopeptides: A Benchmarking Study.Journal of chemical theory and computation · 2024Article
- Protein Association in Solution: Statistical Mechanical Modeling.Biomolecules · 2023Review
- Atomistic modeling of liquid-liquid phase equilibrium explains dependence of critical temperature on γ-crystallin sequence.Communications biology · 2023Article
- Atomistic Modeling of Liquid-Liquid Phase Equilibrium Explains Dependence of Critical Temperature on γ-Crystallin Sequence.bioRxiv : the preprint server for biology · 2023Article
- Conformational Changes and Drivers of Monoclonal Antibody Liquid-Liquid Phase Separation.Journal of pharmaceutical sciences · 2023Article
- Ion-specific binding of cations to the carboxylate and of anions to the amide of alanylalanine.Communications chemistry · 2022Article
- The influence of excipients on the viscosity of monoclonal antibody solutions.Journal of molecular liquids · 2022Article
- Evidence for a semisolid phase state of aerosols and droplets relevant to the airborne and surface survival of pathogens.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The Protein Folding Problem: The Role of Theory.Journal of molecular biology · 2021Review
- Smartphone-Enabled Quantification of Potassium in Blood Plasma.Sensors (Basel, Switzerland) · 2021Article
- Effect of Buffer on Protein Stability in Aqueous Solutions: A Simple Protein Aggregation Model.The journal of physical chemistry. B · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Protein aggregation is broadly important in diseases and in formulations of biological drugs. Here, we develop a theoretical model for reversible protein-protein aggregation in salt solutions. We treat proteins as hard spheres having square-well-energy binding sites, using Wertheim's thermodynamic perturbation theory. The necessary condition required for such modeling to be realistic is that proteins in solution during the experiment remain in their compact form. Within this limitation our model gives accurate liquid-liquid coexistence curves for lysozyme and γ IIIa-crystallin solutions in respective buffers. It provides good fits to the cloud-point curves of lysozyme in buffer-salt mixtures as a function of the type and concentration of salt. It than predicts full coexistence curves, osmotic compressibilities, and second virial coefficients under such conditions. This treatment may also be relevant to protein crystallization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.