Evidence map›Paper›PMID 25963149›Full record

ArticlePlant physiology2015

Down-Regulating the Expression of 53 Soybean Transcription Factor Genes Uncovers a Role for SPEECHLESS in Initiating Stomatal Cell Lineages during Embryo Development.

John Danzer, Eric Mellott, Anhthu Q Bui, Brandon H Le, Patrick Martin, Meryl Hashimoto, Jeanett Perez-Lesher, Min Chen, Julie M Pelletier, David A Somers and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
5.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 43 citations in OpenAlex.

  1. Article
  2. Conservation and Divergence ofPlants (Basel, Switzerland) · 2025
    Article
  3. Candidate genes at the Rmi1 locus for resistance to Meloidogyne incognita in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025
    Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Dissecting the cellular architecture and genetic circuitry of the soybean seed.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  9. A spatially resolved multiomic single-cell atlas of soybean development.bioRxiv : the preprint server for biology · 2024
    Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Plants (Basel, Switzerland) · 2021
    Article
  17. Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

John DanzerMonsanto Company, Agracetus Campus, Middleton, Wisconsin 53562 (J.D., E.M., P.M., J.P.-L., D.A.S);Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095 (A.Q.B., B.H.L., M.C., R.B.G.); andDepartment of Plant Biology, University of California, Davis, California 95616 (M.H., J.M.P., J.J.H.).
Eric MellottMonsanto Company, Agracetus Campus, Middleton, Wisconsin 53562 (J.D., E.M., P.M., J.P.-L., D.A.S);Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095 (A.Q.B., B.H.L., M.C., R.B.G.); andDepartment of Plant Biology, University of California, Davis, California 95616 (M.H., J.M.P., J.J.H.).
Anhthu Q BuiMonsanto Company, Agracetus Campus, Middleton, Wisconsin 53562 (J.D., E.M., P.M., J.P.-L., D.A.S);Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095 (A.Q.B., B.H.L., M.C., R.B.G.); andDepartment of Plant Biology, University of California, Davis, California 95616 (M.H., J.M.P., J.J.H.).
Brandon H LeMonsanto Company, Agracetus Campus, Middleton, Wisconsin 53562 (J.D., E.M., P.M., J.P.-L., D.A.S);Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095 (A.Q.B., B.H.L., M.C., R.B.G.); andDepartment of Plant Biology, University of California, Davis, California 95616 (M.H., J.M.P., J.J.H.).
Patrick MartinMonsanto Company, Agracetus Campus, Middleton, Wisconsin 53562 (J.D., E.M., P.M., J.P.-L., D.A.S);Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095 (A.Q.B., B.H.L., M.C., R.B.G.); andDepartment of Plant Biology, University of California, Davis, California 95616 (M.H., J.M.P., J.J.H.).
Meryl HashimotoMonsanto Company, Agracetus Campus, Middleton, Wisconsin 53562 (J.D., E.M., P.M., J.P.-L., D.A.S);Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095 (A.Q.B., B.H.L., M.C., R.B.G.); andDepartment of Plant Biology, University of California, Davis, California 95616 (M.H., J.M.P., J.J.H.).
Jeanett Perez-LesherMonsanto Company, Agracetus Campus, Middleton, Wisconsin 53562 (J.D., E.M., P.M., J.P.-L., D.A.S);Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095 (A.Q.B., B.H.L., M.C., R.B.G.); andDepartment of Plant Biology, University of California, Davis, California 95616 (M.H., J.M.P., J.J.H.).
Min ChenMonsanto Company, Agracetus Campus, Middleton, Wisconsin 53562 (J.D., E.M., P.M., J.P.-L., D.A.S);Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095 (A.Q.B., B.H.L., M.C., R.B.G.); andDepartment of Plant Biology, University of California, Davis, California 95616 (M.H., J.M.P., J.J.H.).
Julie M PelletierMonsanto Company, Agracetus Campus, Middleton, Wisconsin 53562 (J.D., E.M., P.M., J.P.-L., D.A.S);Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095 (A.Q.B., B.H.L., M.C., R.B.G.); andDepartment of Plant Biology, University of California, Davis, California 95616 (M.H., J.M.P., J.J.H.).
David A SomersMonsanto Company, Agracetus Campus, Middleton, Wisconsin 53562 (J.D., E.M., P.M., J.P.-L., D.A.S);Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095 (A.Q.B., B.H.L., M.C., R.B.G.); andDepartment of Plant Biology, University of California, Davis, California 95616 (M.H., J.M.P., J.J.H.).
Robert B GoldbergMonsanto Company, Agracetus Campus, Middleton, Wisconsin 53562 (J.D., E.M., P.M., J.P.-L., D.A.S);Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095 (A.Q.B., B.H.L., M.C., R.B.G.); andDepartment of Plant Biology, University of California, Davis, California 95616 (M.H., J.M.P., J.J.H.) jjharada@ucdavis.edu bobg@ucla.edu.
John J HaradaMonsanto Company, Agracetus Campus, Middleton, Wisconsin 53562 (J.D., E.M., P.M., J.P.-L., D.A.S);Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095 (A.Q.B., B.H.L., M.C., R.B.G.); andDepartment of Plant Biology, University of California, Davis, California 95616 (M.H., J.M.P., J.J.H.) jjharada@ucdavis.edu bobg@ucla.edu.
Monsanto (United States) · USUniversity of California, Los Angeles · USUniversity of California, Davis · US

Funding

Training Grant in Genomic Analysis and InterpretationT32HG002536 · NHGRI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Valerie A Arboleda, Harold Pimentel · 2002 to 2026
$8.6M
NHGRI NIH HHS T32 HG002536
6 · The paper itself

Abstract

We used an RNA interference screen to assay the function of 53 transcription factor messenger RNAs (mRNAs) that accumulate specifically within soybean (Glycine max) seed regions, subregions, and tissues during development. We show that basic helix-loop-helix (bHLH) transcription factor genes represented by Glyma04g41710 and its paralogs are required for the formation of stoma in leaves and stomatal precursor complexes in mature embryo cotyledons. Phylogenetic analysis indicates that these bHLH transcription factor genes are orthologous to Arabidopsis (Arabidopsis thaliana) SPEECHLESS (SPCH) that initiate asymmetric cell divisions in the leaf protoderm layer and establish stomatal cell lineages. Soybean SPCH (GmSPCH) mRNAs accumulate primarily in embryo, seedling, and leaf epidermal layers. Expression of Glyma04g41710 under the control of the SPCH promoter rescues the Arabidopsis spch mutant, indicating that Glyma04g41710 is a functional ortholog of SPCH. Developing soybean embryos do not form mature stoma, and stomatal differentiation is arrested at the guard mother cell stage. We analyzed the accumulation of GmSPCH mRNAs during soybean seed development and mRNAs orthologous to MUTE, FAMA, and inducer of C-repeat/dehydration responsive element-binding factor expression1/scream2 that are required for stoma formation in Arabidopsis. The mRNA accumulation patterns provide a potential explanation for guard mother cell dormancy in soybean embryos. Our results suggest that variation in the timing of bHLH transcription factor gene expression can explain the diversity of stomatal forms observed during plant development.

Indexed as

Genes, PlantArabidopsis ProteinsBasic Helix-Loop-Helix ProteinsCell LineageDown-RegulationGene Expression ProfilingGene Expression Regulation, DevelopmentalGene Expression Regulation, PlantGerminationGlycine maxHomozygotePlant DevelopmentPlant LeavesPlant ProteinsPlants, Genetically ModifiedPlant StomataArabidopsis ProteinsBasic Helix-Loop-Helix ProteinsPlant ProteinsRNA, MessengerSPEECHLESS protein, Arabidopsis

Identifiers

PMID25963149
PMCPMC4741349
OpenAlexW1496001836

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.