ArticleVeterinary dermatology2015
Expression and localization of epithelial stem cell and differentiation markers in equine skin, eye and hoof.
Article in Veterinary dermatology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- Witch Nails (Krt90whnl): A spontaneous mouse mutation affecting nail growth and development.PloS one · 2022Article
- Photodynamic therapy accelerates skin wound healing through promoting re-epithelialization.Burns & trauma · 2021Article
- The capacity of goat epidermal adult stem cells to reconstruct the damaged ocular surface of total LSCD and activate corneal genetic programs.Journal of molecular histology · 2020Article
- Interleukin-17A pathway target genes are upregulated in Equus caballus supporting limb laminitis.PloS one · 2020Article
- Morphological and immunohistochemical characteristics of the equine corneal epithelium.Veterinary ophthalmology · 2019Article
- Ultrastructural morphology is distinct among primary progenitor cell isolates from normal, inflamed, and cryopreserved equine hoof tissue and CD105In vitro cellular & developmental biology. Animal · 2019Article
- Molecular markers for corneal epithelial cells in larval vs. adult Xenopus frogs.Experimental eye research · 2019Article
- Physics of animal health: on the mechano-biology of hoof growth and form.Journal of the Royal Society, Interface · 2019Article
- Article
- The expression of equine keratins K42 and K124 is restricted to the hoof epidermal lamellae of Equus caballus.PloS one · 2019Article
- Keratinocyte Growth Factor (KGF) Modulates Epidermal Progenitor Cell Kinetics through Activation of p63 in Middle Ear Cholesteatoma.Journal of the Association for Research in Otolaryngology : JARO · 2018Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe limited characterization of equine skin, eye and hoof epithelial stem cell (ESC) and differentiation markers impedes the investigation of the physiology and pathophysiology of these tissues. HYPOTHESIS/
objectivesTo characterize ESC and differentiation marker expression in epithelial tissues of the equine eye, haired skin and hoof capsule.
methodsIndirect immunofluorescence microscopy and immunoblotting were used to detect expression and tissue localization of keratin (K) isoforms K3, K10, K14 and K124, the transcription factor p63 (a marker of ESCs) and phosphorylated p63 [pp63; a marker of ESC transition to transit-amplifying (TA) cell] in epithelial tissues of the foot (haired skin, hoof coronet and hoof lamellae) and the eye (limbus and cornea).
resultsExpression of K14 was restricted to the basal layer of epidermal lamellae and to basal and adjacent suprabasal layers of the haired skin, coronet and corneal limbus. Coronary and lamellar epidermis was negative for both K3 and K10, which were expressed in the cornea/limbus epithelium and haired skin epidermis, respectively. Variable expression of p63 with relatively low to high levels of phosphorylation was detected in individual basal and suprabasal cells of all epithelial tissues examined.
conclusionsTo the best of the author's knowledge, this is the first report of the characterization of tissue-specific keratin marker expression and the localization of putative epithelial progenitor cell populations, including ESCs (high p63 expression with low pp63 levels) and TA cells (high expression of both p63 and pp63), in the horse. These results will aid further investigation of epidermal and corneal epithelial biology and regenerative therapies in horses.
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